2023-05-01 ミネソタ大学
この技術は、Nano Lettersで紹介されています。研究者たちは、CWDの鹿に焦点を当てた論文であるが、目的は神経変性疾患の広範囲にわたってこの技術を拡大することであり、特にアルツハイマー病とパーキンソン病を対象としています。
彼らは、神経変性疾患の治療を遅らせることができる治療薬の投与に十分な時間を提供するために、バイオマーカーを早期に検出するための超感度で強力な診断技術を開発したいと考えています。
<関連情報>
- https://cse.umn.edu/college/news/u-m-researchers-develop-technique-rapid-detection-neurodegenerative-diseases
- https://pubs.acs.org/doi/10.1021/acs.nanolett.3c01001
ミスフォールドタンパク質のナノ粒子強化RT-QUIC(Nano-QuIC)診断法 Nanoparticle-Enhanced RT-QuIC (Nano-QuIC) Diagnostic Assay for Misfolded Proteins
Peter R. Christenson, Manci Li, Gage Rowden, Peter A. Larsen and Sang-Hyun Oh
Nano Letters Published:April 26, 2023
DOI:https://doi.org/10.1021/acs.nanolett.3c01001
Abstract
Misfolded proteins associated with various neurodegenerative diseases often accumulate in tissues or circulate in biological fluids years before the clinical onset, thus representing ideal diagnostic targets. Real-time quaking-induced conversion (RT-QuIC), a protein-based seeded-amplification assay, holds great potential for early disease detection, yet challenges remain for routine diagnostic application. Chronic Wasting Disease (CWD), associated with misfolded prion proteins of cervids, serves as an ideal model for evaluating new RT-QuIC methodologies. In this study, we investigate the previously untested hypothesis that incorporating nanoparticles into RT-QuIC assays can enhance their speed and sensitivity when applied to biological samples. We show that adding 50 nm silica nanoparticles to RT-QuIC experiments (termed Nano-QuIC) for CWD diagnostics greatly improves the performance by reducing detection times 2.5-fold and increasing sensitivity 10-fold by overcoming the effect of inhibitors in complex tissue samples. Crucially, no false positives were observed with these 50 nm silica nanoparticles, demonstrating the enhanced reliability and potential for diagnostic application of Nano-QuIC in detecting misfolded proteins.
