2025-09-05 マックス・プランク研究所
Fluorescence image of a mouse hippocampal slice. Synapses are shown in green, and blue marks the cell bodies of neurons.
© Max Planck Institute for Brain Research / R. Ray
<関連情報>
- https://www.mpg.de/25328445/a-molecular-atlas-of-the-hippocampus-mapping-rnas-and-proteins-at-synaptic-resolution
- https://www.nature.com/articles/s41467-025-63119-5
シナプス分解能におけるマウス海馬の統合トランスクリプトーム・プロテオームマップ An integrated transcriptomic and proteomic map of the mouse hippocampus at synaptic resolution
Eva Kaulich,Quinn Waselenchuk,Nicole Fürst,Kristina Desch,Janus Mosbacher,Elena Ciirdaeva,Marcel Juengling,Roshni Ray,Belquis Nassim-Assir,Georgi Tushev,Julian D. Langer & Erin M. Schuman
Nature Communications Published:26 August 2025
DOI:https://doi.org/10.1038/s41467-025-63119-5
Abstract
Understanding the brain’s molecular diversity requires spatially resolved maps of transcripts and proteins across regions and compartments. Here, we performed deep spatial molecular profiling of the mouse hippocampus, combining microdissection of 3 subregions and 4 strata with fluorescence-activated synaptosome sorting, transcriptomics, and proteomics. This approach revealed thousands of locally enriched molecules spanning diverse receptor, channel, metabolic, and adhesion families. Integration of transcriptome and proteome data highlighted proteins tightly linked to or decoupled from mRNA availability, in part due to protein half-life differences. Incorporation of translatome data identified roles for protein trafficking versus local translation in establishing compartmental organization of pyramidal neurons, with distal dendrites showing increased reliance on local protein synthesis. Classification of CA1 synapses revealed contributions from kinases, cytoskeletal elements, and adhesion molecules in defining synaptic specificity. Together, this study provides a molecular atlas of the hippocampus and its synapses (syndive.org), and offers insights into spatial transcript-protein relationships.
