カリフォルニア大学リバーサイド校が主導する研究は、公衆衛生を守るための規制の必要性を強調しています。 UC Riverside-led study highlights need for regulation to protect public health
2022-08-02 カリフォルニア大学リバーサイド校(UCR)
研究チームは、ガスクロマトグラフと質量分析を用いて、Puff ECの液体に含まれる126種類の香料化学物質を特定し、これらの化学物質がヒト気管支上皮細胞に対してどの程度の毒性を持つかを検証しました。その結果、使い捨てのパフECに含まれる化学物質が高濃度で、細胞毒性、つまり生きている細胞に対して毒性があることが、学術誌「Chemical Research in Toxicology」に報告されました。
<関連情報>
- https://news.ucr.edu/articles/2022/08/02/coolants-puff-electronic-cigarettes-present-health-hazard
- https://pubs.acs.org/doi/10.1021/acs.chemrestox.1c00423
Disposable Puff Bar Electronic Cigarettes: Chemical Composition and Toxicity of E-liquids and a Synthetic Coolant 使い捨てパフバー型電子タバコ。電子タバコ用リキッドと合成清涼飲料水の化学組成と毒性について
Esther E. Omaiye, Wentai Luo, Kevin J. McWhirter, James F. Pankow, and Prue Talbot
Chemical Research in Toxicology Published:July 18, 2022
DOI:https://doi.org/10.1021/acs.chemrestox.1c00423
Abstract
The popularity of disposable fourth-generation electronic cigarettes (ECs) among young adults and adolescents has been increasing since the ban on flavored cartridge EC products such as JUUL. Although the constituents and toxicity of some cartridge-based fourth-generation ECs, such as JUUL, have been studied, limited data exist for other disposable ECs such as Puff. The purpose of this study was to determine flavor chemicals, synthetic coolants, and nicotine concentrations in 16 disposable Puff devices, evaluate the cytotoxicity of the different flavors from the Puff brand using in vitro assays, and investigate the health risks of synthetic coolants in EC products. Gas chromatography/mass spectrometry was used to identify and quantify chemicals in Puff EC fluids. One hundred and twenty-six flavor chemicals were identified in Puff fluids, and 16 were >1 mg/mL. WS-23 (2-isopropyl-N,2,3-trimethylbutyramide) was present in all products, and concentrations ranged from 0.8 to 45.1 mg/mL. WS-3 (N-ethyl-p-menthane-3-carboxamide) concentrations ranged from 1.5 to 16.4 mg/mL in 6/16 products. Nicotine concentrations ranged from 40.6 to 52.4 (average 44.8 mg/mL). All unvaped fluids were cytotoxic at dilutions between 0.1 and 10% in the MTT and neutral red uptake assays when tested with BEAS-2B lung epithelial cells. The cytotoxicity of Puff fluids was highly correlated with total chemical concentrations, nicotine, WS-23, both synthetic coolants, and synthetic coolants plus ethyl maltol. Lower concentrations of WS-23 than those in the fluids adversely affected cell growth and morphology. Concentrations of synthetic coolants exceeded levels used in consumer products. The margin of exposure data showed that WS-3 and WS-23 concentrations were high enough in Puff products to present a health hazard. Our study demonstrates that disposable Puff ECs have high levels of cytotoxic chemicals. The data support the regulation of flavor chemicals and synthetic coolants in ECs to limit potentially harmful health effects.
