2024-01-24 韓国基礎科学研究院(IBS)
◆これにより、LDsの機能理解が進み、肥満や非アルコール性脂肪肝疾患の診断と治療に応用される見通し。2C-IPMは蛍光染料を必要とせず、赤外線吸収の変化を検出して中性脂肪を直接モニタリングし、細胞内での中性脂肪合成を長期間観察可能。
<関連情報>
- https://www.ibs.re.kr/cop/bbs/BBSMSTR_000000000738/selectBoardArticle.do
- https://pubs.rsc.org/en/content/articlelanding/2024/sc/d3sc04705a
2色赤外光熱顕微鏡を用いた生きたヒトがん細胞の脂質滴における中性脂質の合成モニタリング Monitoring the synthesis of neutral lipids in lipid droplets of living human cancer cells using two-color infrared photothermal microscopy
Chanjong Park, Jong Min Lim, Seok-Cheol Hong and Minhaeng Cho
Chemical Science Published:01 Dec 2023
DOI:https://doi.org/10.1039/D3SC04705A
Abstract
There has been growing interest in the functions of lipid droplets (LDs) due to recent discoveries regarding their diverse roles. These functions encompass lipid metabolism, regulation of lipotoxicity, and signaling pathways that extend beyond their traditional role in energy storage. Consequently, there is a need to examine the molecular dynamics of LDs at the subcellular level. Two-color infrared photothermal microscopy (2C-IPM) has proven to be a valuable tool for elucidating the molecular dynamics occurring in LDs with sub-micrometer spatial resolution and molecular specificity. In this study, we employed the 2C-IPM to investigate the molecular dynamics of LDs in both fixed and living human cancer cells (U2OS cells) using the isotope labeling method. We investigated the synthesis of neutral lipids occurring in individual LDs over time after exposing the cells to excess saturated fatty acids while simultaneously comparing inherent lipid contents in LDs. We anticipate that these research findings will reveal new opportunities for studying lesser-known biological processes within LDs and other subcellular organelles.
