革新的な顕微鏡技術で細胞内の脂質合成の秘密を解明(Innovative Microscopy Technique Reveals Secrets of Lipid Synthesis Inside Cells)

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2024-01-24 韓国基礎科学研究院(IBS)

◆韓国のIBS分子スペクトロスコピーとダイナミクスセンターのCHO Minhaengセンター長率いる研究チームが、新技術「Two-Color Infrared Photothermal Microscopy(2C-IPM)」を開発。これは、生きた細胞のリン脂質滴内の中性脂肪を調査するための画期的な顕微鏡で、同位体ラベリングを組み合わせて中性脂肪の合成を詳細にモニタリング可能。
◆これにより、LDsの機能理解が進み、肥満や非アルコール性脂肪肝疾患の診断と治療に応用される見通し。2C-IPMは蛍光染料を必要とせず、赤外線吸収の変化を検出して中性脂肪を直接モニタリングし、細胞内での中性脂肪合成を長期間観察可能。

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2色赤外光熱顕微鏡を用いた生きたヒトがん細胞の脂質滴における中性脂質の合成モニタリング Monitoring the synthesis of neutral lipids in lipid droplets of living human cancer cells using two-color infrared photothermal microscopy

Chanjong Park, Jong Min Lim,  Seok-Cheol Hong   and  Minhaeng Cho
Chemical Science  Published:01 Dec 2023
DOI:https://doi.org/10.1039/D3SC04705A

Abstract

There has been growing interest in the functions of lipid droplets (LDs) due to recent discoveries regarding their diverse roles. These functions encompass lipid metabolism, regulation of lipotoxicity, and signaling pathways that extend beyond their traditional role in energy storage. Consequently, there is a need to examine the molecular dynamics of LDs at the subcellular level. Two-color infrared photothermal microscopy (2C-IPM) has proven to be a valuable tool for elucidating the molecular dynamics occurring in LDs with sub-micrometer spatial resolution and molecular specificity. In this study, we employed the 2C-IPM to investigate the molecular dynamics of LDs in both fixed and living human cancer cells (U2OS cells) using the isotope labeling method. We investigated the synthesis of neutral lipids occurring in individual LDs over time after exposing the cells to excess saturated fatty acids while simultaneously comparing inherent lipid contents in LDs. We anticipate that these research findings will reveal new opportunities for studying lesser-known biological processes within LDs and other subcellular organelles.

革新的な顕微鏡技術で細胞内の脂質合成の秘密を解明(Innovative Microscopy Technique Reveals Secrets of Lipid Synthesis Inside Cells)

 

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