2024-11-15 タフツ大学
<関連情報>
- https://now.tufts.edu/2024/11/15/study-challenges-assumptions-about-how-tuberculosis-bacteria-grow
- https://www.nature.com/articles/s41564-024-01846-z
結核菌の細胞周期の単一細胞イメージングにより、直線的かつ不均一な増殖が明らかになる Single-cell imaging of the Mycobacterium tuberculosis cell cycle reveals linear and heterogenous growth
Eun Seon Chung,Prathitha Kar,Maliwan Kamkaew,Ariel Amir & Bree B. Aldridge
Nature Microbiology Published:15 November 2024
DOI:https://doi.org/10.1038/s41564-024-01846-z
Abstract
Difficulties in antibiotic treatment of Mycobacterium tuberculosis (Mtb) are partly thought to be due to heterogeneity in growth. Although the ability of bacterial pathogens to regulate growth is crucial to control homeostasis, virulence and drug responses, single-cell growth and cell cycle behaviours of Mtb are poorly characterized. Here we use time-lapse, single-cell imaging of Mtb coupled with mathematical modelling to observe asymmetric growth and heterogeneity in cell size, interdivision time and elongation speed. We find that, contrary to Mycobacterium smegmatis, Mtb initiates cell growth not only from the old pole but also from new poles or both poles. Whereas most organisms grow exponentially at the single-cell level, Mtb has a linear growth mode. Our data show that the growth behaviour of Mtb diverges from that of model bacteria, provide details into how Mtb grows and creates heterogeneity and suggest that growth regulation may also diverge from that in other bacteria.
