2025-08-28 理化学研究所,バイオ産業情報化コンソーシアム,東京大学
DDX3XがmRNAを選択的に認識し、翻訳制御を行う分子機構の模式図
<関連情報>
- https://www.riken.jp/press/2025/20250828_2/index.html
- https://www.nature.com/articles/s41467-025-62806-7
DEAD-box RNAヘリカーゼDDX3XのN末端固有無秩序領域が選択的RNA認識に果たす調節的役割 Regulatory role of the N-terminal intrinsically disordered region of the DEAD-box RNA helicase DDX3X in selective RNA recognition
Yuki Toyama,Koh Takeuchi & Ichio Shimada
Nature Communications Published:28 August 2025
DOI:https://doi.org/10.1038/s41467-025-62806-7
Abstract
DDX3X, a member of the DEAD-box RNA helicase family, plays a central role in the translational regulation of gene expression through its unwinding activity toward complex RNA structures in messenger RNAs (mRNAs). Although DDX3X is known to selectively stimulate the translation of a subset of genes, a specific sequence motif has not been identified; thus, the molecular mechanism underlying this selectivity remains elusive. Using solution nuclear magnetic resonance (NMR) spectroscopy, we demonstrate that the N-terminal intrinsically disordered region (IDR) of DDX3X plays a critical role in the binding and unwinding of structured RNAs. We propose that the selectivity toward target transcripts is mediated by its preferential binding to structured motifs, particularly the G-quadruplex structure, through arginine-rich segments within the N-terminal IDR. Our results provide a molecular basis for understanding translational regulation by DDX3X and highlight the remarkable role of the flexible IDR in controlling the cellular translational landscape.
