2023-10-18 カロリンスカ研究所(KI)
A chip enables micro-organs to be positioned in the eye
◆眼は免疫細胞が反応しないため、移植物の視覚的な研究が可能であり、デバイスは眼の前房に機械的に固定され、マウスでの試験では数か月間位置を維持し、微小臓器が宿主の血管と迅速に統合し正常に機能しました。
◆この技術は、細胞療法の発展において、細胞移植の成功を確認するための侵襲的な方法が不要であるため、細胞の長期的な監視に適しています。将来的には、統合電子機器や薬物放出などの高度なデバイス機能の統合と使用が可能となります。
<関連情報>
- https://news.ki.se/eye-implant-may-be-used-to-treat-diabetes
- https://onlinelibrary.wiley.com/doi/10.1002/adma.202306686
3Dプリンティングされたバイオハイブリッド微細構造により、眼球前房における微小組織の移植と脈管形成が可能になる 3D-Printed Biohybrid Microstructures Enable Transplantation and Vascularization of Microtissues in the Anterior Chamber of the Eye
Hanie Kavand, Montse Visa, Martin Köhler, Wouter van der Wijngaart, Per-Olof Berggren, Anna Herland
Advanced Materials Published: 10 October 2023
DOI:https://doi.org/10.1002/adma.202306686
Abstract
Hybridizing biological cells with man-made sensors enable the detection of a wide range of weak physiological responses with high specificity. The anterior chamber of the eye (ACE) is an ideal transplantation site due to its ocular immune privilege and optical transparency, which enable superior non-invasive longitudinal analyses of cells and microtissues. Engraftment of biohybrid microstructures in the ACE might, however, be affected by the pupillary response and dynamics. Here, sutureless transplantation of biohybrid microstructures, 3D printed in IP-Visio photoresin, containing a precisely localized pancreatic islet to the ACE of mice is presented. The biohybrid microstructures allow mechanical fixation in the ACE, independent of iris dynamics. After transplantation, islets in the microstructures successfully sustain their functionality for over 20 weeks and become vascularized despite physical separation from the vessel source (iris) and immersion in a low-viscous liquid (aqueous humor) with continuous circulation and clearance. This approach opens new perspectives in biohybrid microtissue transplantation in the ACE, advancing monitoring of microtissue-host interactions, disease modeling, treatment outcomes, and vascularization in engineered tissues.
