2025-07-07 早稲田大学
図1 植物刺入型多酵素センサシステムの開発
<関連情報>
- https://www.waseda.jp/inst/research/news/81393
- https://www.sciencedirect.com/science/article/pii/S0956566325005482?via%3Dihub
気孔からのスクロース取り込みをリアルタイムでモニタリングする植物挿入型マルチ酵素バイオセンサー A plant-insertable multi-enzyme biosensor for the real-time monitoring of stomatal sucrose uptake
Shiqi Wu, Wakutaka Nakagawa, Yuki Mori, Saman Azhari, Gábor Méhes, Yuta Nishina, Tomonori Kawano, Takeo Miyake
Biosensors and Bioelectronics Available online: 8 June 2025
DOI:https://doi.org/10.1016/j.bios.2025.117674
Highlights
- Needle-type multi-enzyme sensor for real-time sucrose detection in plant tissues.
- Demonstrates light-dependent stomatal uptake of water and sucrose in Japanese cedar.
- Reveals higher night-time sucrose transport in strawberry guava stems.
- Supports precision agriculture with real-time tracking of plant sugar dynamics.
Abstract
Monitoring sucrose transport in plants is essential for understanding plant physiology and improving agricultural practices, yet effective sensors for continuous and real-time in-vivo monitoring are lacking. In this study, we developed a plant-insertable sucrose sensor capable of real-time sucrose concentration monitoring and demonstrated its application as a useful tool for plant research by monitoring the sugar-translocating path from leaves to the lower portion of plants through the stem in living plants. The biosensor consists of a bilirubin oxidase-based biocathode and a needle-type bioanode integrating glucose oxidase, invertase, and mutarotase, with the two electrodes separated by an agarose gel for ionic connection. The sensor exhibits a sensitivity of 6.22 μA mM-1 cm-2, a limit of detection of 100 μM, a detection range up to 60 mM, and a response time of 90 s at 100 μM sucrose. Additionally, the sensor retained 86 % of its initial signal after 72 h of continuous measurement. Day-night monitoring from the biosensor inserted in strawberry guava (Psidium cattleianum) showed higher sucrose transport activity at night, following well the redistribution of photosynthetically produced sugars. In addition, by monitoring the forced translocation of sucrose dissolved in the stable isotopically labeled water, we demonstrated that a young seedling of Japanese cedar known as Sugi (Cryptomeria japonica) can absorb and transport both water and sucrose through light-dependently opened stomata, which is the recently revealed path for liquid uptake by higher plants. These findings highlight the potential of our sensor for studying dynamic plant processes and its applicability in real-time monitoring of sugar transport under diverse environmental conditions.
