2026-02-18 デューク大学
Microscopic results from the testing of various materials and how they stand up to bacterial infections. Natural tissue (bottom) barely sees any bacterial growth (neon green), whereas Alginate (top) has a lot of bacterial growth, and GelMA (middle) is susceptible to bacterial penetration in a specific place.
<関連情報>
- https://pratt.duke.edu/news/complexity-key-to-preventing-infection-after-heart-surgery/
- https://onlinelibrary.wiley.com/doi/10.1002/jbm.a.70050
3Dプリント材料における大腸菌および黄色ブドウ球菌の転座に関するin vitro評価 In Vitro Evaluation of Escherichia coli and Staphylococcus aureus Translocation in 3D Printed Material
Ashma Sharma, Joshua Prince, A-Andrew D. Jones III
Journal of Biomedical Materials Part A Published: 16 February 2026
DOI:https://doi.org/10.1002/jbm.a.70050
ABSTRACT
Vascular graft infection is a rare but life-threatening condition, primarily occurring after 30 days post-surgery. Meta-analysis has shown that antimicrobial coatings on graft materials do not prevent these infections. Moreover, infections still occurs even though studies have shown that there is no bacterial proliferation or bacterial penetration of common vascular graft material. The time frame of infection, meta-analysis, and in situ studies suggest that bacteria present at the suture site are introduced into the surrounding tissue or that systemically circulating bacteria may be surviving, proliferating, diffusing slowly, and evading host immune defense in synthetic vascular grafts. De novo vascular graft materials, such as tissue-engineered vascular graft material and decellularized vasculature may provide an in situ platform for studying survival, proliferation, and diffusion in tissue and tissue-like materials. In this study, we used confocal microscopy to image the penetration depth of bacteria over time as a proxy for the diffusion of Staphylococcus aureus and Escherichia coli into alginate, GelMA, and decellularized porcine vascular tissue. We quantified viable bacteria breakthrough as a function of biomaterial type. We found that the penetration depth over time was similar in all three biomaterials, however E. coli broke through much less from tissue than from engineered materials, while S. aureus had higher breakthrough in the GelMa but otherwise equal rates. These results point to the possibility of interstitial growth control relative to surface coatings as a future target for engineering infection resistance in engineered vascular grafts.
