2023-08-09 エディンバラ大学
◆エジンバラ大学の研究チームが開発し、血管や消化管などの組織モデルを作成可能。これにより、動物実験を減少させつつ、薬物開発などに貢献。液体を高速で回転するチューブ内で細胞を均一に分布させ、層を形成。臨床試験が必要であり、人間の移植に使用される前にはさらなる研究が必要。
<関連情報>
- https://www.ed.ac.uk/news/2023/new-tech-is-step-towards-lab-grown-blood-vessels
- https://iopscience.iop.org/article/10.1088/1758-5090/ace2ed
回転内部流動層工学による層状組織バイオファブリケーション Stratified tissue biofabrication by rotational internal flow layer engineering
Ian Holland, Wenmiao Shu and Jamie A Davies
Biofabrication Published 19 July 2023
DOI:10.1088/1758-5090/ace2ed
Abstract
The bioassembly of layered tissue that closely mimics human histology presents challenges for tissue engineering. Existing bioprinting technologies lack the resolution and cell densities necessary to form the microscale cell-width layers commonly observed in stratified tissue, particularly when using low-viscosity hydrogels, such as collagen. Here we present rotational internal flow layer engineering (RIFLE), a novel, low-cost biofabrication technology for assembling tuneable, multi-layered tissue-like structures. Using high-speed rotating tubular moulds, small volumes of cell-laden liquids added to the inner surface were transitioned into thin layers and gelled, progressively building macroscale tubes composed of discrete microscale strata with thicknesses a function of rotational speed. Cell encapsulation enabled the patterning of high-density layers (108 cells ml-1) into heterogenous constructs. RIFLE versatility was demonstrated through tunica media assembly, encapsulating human smooth muscle cells in cell-width (12.5 µm) collagen layers. Such deposition of discrete microscale layers, facilitates the biofabrication of composite structures mimicking the nature of native stratified tissue. This enabling technology has the potential to allow researchers to economically create a range of representative layered tissue.
