新しい分子ツールキットが酵母コミュニティーからの有用分子生産を促進(New molecular toolkit boosts useful molecule production from yeast communities)

2023-02-07 インペリアル・カレッジ・ロンドン(ICL)

<関連情報>

• https://www.imperial.ac.uk/news/251334/new-molecular-toolkit-boosts-useful-molecule/
• https://www.nature.com/articles/s41564-023-01596-4

合成酵母コミュニティー工学のためのクロスフィード株の分子ツールキット A molecular toolkit of cross-feeding strains for engineering synthetic yeast communities

Huadong Peng,Alexander P. S. Darlington,Eric J. South,Hao-Hong Chen,Wei Jiang & Rodrigo Ledesma-Amaro
Nature Microbiology  Published:07 February 2024
DOI:https://doi.org/10.1038/s41564-023-01596-4

Abstract

Engineered microbial consortia often have enhanced system performance and robustness compared with single-strain biomanufacturing production platforms. However, few tools are available for generating co-cultures of the model and key industrial host Saccharomyces cerevisiae. Here we engineer auxotrophic and overexpression yeast strains that can be used to create co-cultures through exchange of essential metabolites. Using these strains as modules, we engineered two- and three-member consortia using different cross-feeding architectures. Through a combination of ensemble modelling and experimentation, we explored how cellular (for example, metabolite production strength) and environmental (for example, initial population ratio, population density and extracellular supplementation) factors govern population dynamics in these systems. We tested the use of the toolkit in a division of labour biomanufacturing case study and show that it enables enhanced and tuneable antioxidant resveratrol production. We expect this toolkit to become a useful resource for a variety of applications in synthetic ecology and biomanufacturing.