2024-09-11 韓国基礎科学研究院(IBS)
<関連情報>
- https://www.ibs.re.kr/cop/bbs/BBSMSTR_000000000738/selectBoardArticle.do?nttId=25015&pageIndex=1&searchCnd=&searchWrd=
- https://www.nature.com/articles/s41583-024-00851-9
シナプスの標識化と選択的操作の進歩 Advances in the labelling and selective manipulation of synapses
Binod Timalsina,Sangkyu Lee & Bong-Kiun Kaang
Nature Reviews Neuroscience Published:22 August 2024
DOI:https://doi.org/10.1038/s41583-024-00851-9
Abstract
Synapses are highly specialized neuronal structures that are essential for neurotransmission, and they are dynamically regulated throughout the lifetime. Although accumulating evidence indicates that these structures are crucial for information processing and storage in the brain, their precise roles beyond neurotransmission are yet to be fully appreciated. Genetically encoded fluorescent tools have deepened our understanding of synaptic structure and function, but developing an ideal methodology to selectively visualize, label and manipulate synapses remains challenging. Here, we provide an overview of currently available synapse labelling techniques and describe their extension to enable synapse manipulation. We categorize these approaches on the basis of their conceptual bases and target molecules, compare their advantages and limitations and propose potential modifications to improve their effectiveness. These methods have broad utility, particularly for investigating mechanisms of synaptic function and synaptopathy.
