2024-10-17 スイス連邦工科大学ローザンヌ校(EPFL)
<関連情報>
- https://actu.epfl.ch/news/e-flower-records-neuronal-activity-with-electronic/
- https://www.science.org/doi/10.1126/sciadv.adp8054
e-Flower:脳スフェロイド電気生理学のためのハイドロゲル作動型3D MEA The e-Flower: A hydrogel-actuated 3D MEA for brain spheroid electrophysiology
Eleonora Martinelli, Outman Akouissi, Luca Liebi, Ivan Furfaro, […], and Stéphanie P. Lacour
Science Advances Published:16 Oct 2024
DOI:https://doi.org/10.1126/sciadv.adp8054
Abstract
Traditional microelectrode arrays (MEAs) are limited to measuring electrophysiological activity in two dimensions, failing to capture the complexity of three-dimensional (3D) tissues such as neural organoids and spheroids. Here, we introduce a flower-shaped MEA (e-Flower) that can envelop submillimeter brain spheroids following actuation by the sole addition of the cell culture medium. Inspired by soft microgrippers, its actuation mechanism leverages the swelling properties of a polyacrylic acid hydrogel grafted to a polyimide substrate hosting the electrical interconnects. Compatible with standard electrophysiology recording systems, the e-Flower does not require additional equipment or solvents and is ready to use with preformed 3D tissues. We designed an e-Flower achieving a curvature as low as 300 micrometers within minutes, a value tunable by the choice of reswelling media and hydrogel cross-linker concentration. Furthermore, we demonstrate the ability of the e-Flower to detect spontaneous neural activity across the spheroid surface, demonstrating its potential for comprehensive neural signal recording.
