2024-12-19 韓国基礎科学研究院(IBS)
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- https://www.ibs.re.kr/cop/bbs/BBSMSTR_000000000738/selectBoardArticle.do?nttId=25406&pageIndex=1&searchCnd=&searchWrd=
- https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae1122/7917113
包括的全ゲノム配列決定により、加齢、ミスマッチ修復欠損、テモゾロミド化学療法に関連する変異シグネチャーの起源が明らかになった Comprehensive whole-genome sequencing reveals origins of mutational signatures associated with aging, mismatch repair deficiency and temozolomide chemotherapy
Taejoo Hwang, Lukasz Karol Sitko, Ratih Khoirunnisa, Fernanda Navarro-Aguad, David M Samuel, Hajoong Park, Banyoon Cheon, Luthfiyyah Mutsnaini, Jaewoong Lee, Burçak Otlu …
Nucleic Acids Research Published:05 December 2024
DOI:https://doi.org/10.1093/nar/gkae1122
Graphical Abstract
Abstract
In a comprehensive study to decipher the multi-layered response to the chemotherapeutic agent temozolomide (TMZ), we analyzed 427 genomes and determined mutational patterns in a collection of ∼40 isogenic DNA repair-deficient human TK6 lymphoblast cell lines. We first demonstrate that the spontaneous mutational background is very similar to the aging-associated mutational signature SBS40 and mainly caused by polymerase zeta-mediated translesion synthesis (TLS). MSH2-/- mismatch repair (MMR) knockout in conjunction with additional repair deficiencies uncovers cryptic mutational patterns. We next report how distinct mutational signatures are induced by TMZ upon sequential inactivation of DNA repair pathways, mirroring the acquisition of chemotherapy resistance by glioblastomas. The most toxic adduct induced by TMZ, O6-meG, is directly repaired by the O6-methylguanine-DNA methyltransferase (MGMT). In MGMT-/- cells, MMR leads to cell death and limits mutagenesis. MMR deficiency results in TMZ resistance, allowing the accumulation of ∼105 C > T substitutions corresponding to signature SBS11. Under these conditions, N3-methyladenine (3-meA), processed by base excision repair (BER), limits cell survival. Without BER, 3-meA is read through via error-prone TLS, causing T > A substitutions but not affecting survival. Blocking BER after abasic site formation results in large deletions and TMZ hypersensitization. Our findings reveal potential vulnerabilities of TMZ-resistant tumors.
