2025-03-20 中国科学院(CAS)
◆インプラント関連感染症は術後の重大な課題であり、バイオフィルム内の細菌は抗生物質治療を回避することが知られています。従来の抗菌アプローチは、細菌と健康な細胞の両方に影響を及ぼす可能性がありました。しかし、今回の研究では、金とアルカリ性マグネシウム-鉄混合金属酸化物からなるショットキー異種接合膜を使用し、細菌の呼吸鎖からプロトンと電子を捕捉することで、ATP合成や他の生合成プロセスを阻害し、最終的に細菌の死滅を引き起こすことが示されました。この異種接合膜は、哺乳類細胞のエネルギー代謝には影響を与えず、生体内試験でも安全性と有効性が確認されました。
<関連情報>
- https://english.cas.cn/newsroom/research_news/life/202503/t20250320_908335.shtml
- https://www.science.org/doi/10.1126/sciadv.adt3159
プロトンと電子の授受を妨害することで、抗菌薬飢餓療法が可能になる Interfering with proton and electron transfer enables antibacterial starvation therapy
Ji Tan, Haifeng Zhang, Yisi Liu, Zhenhao Hou, […], and Xuanyong Liu
Science Advances Published:19 Mar 2025
DOI:https://doi.org/10.1126/sciadv.adt3159
Abstract
Implant-associated infections are urgently addressed; however, existing materials are difficult to kill bacteria without damaging cells. Here, we propose an innovative concept of selective antibacterial starvation therapy based on interfering with proton and electron transfer on the bacterial membrane. As a proof-of-principle demonstration, a special Schottky heterojunction film composed of gold and alkaline magnesium-iron mixed metal oxides (Au/MgFe-MMO) was constructed on the titanium implant. Once bacteria contacted this implant, the Au/MgFe-MMO film continuously captured the proton and electron participated in respiratory chain of bacteria to impede their energy metabolism, leading to the deficit of adenosine 5′-triphosphate. Prolonged exposure to this starvation state inhibited numerous biosynthesis processes and triggered severe oxidative stress in bacteria, ultimately leading to their death due to DNA and membrane damage. In addition, this heterojunction film was comfortable for mammalian cells, without inhibiting mitochondrial function. This proposed starvation antibacterial therapy gives a notable perspective in designing biosafe smart antibacterial biomaterials.
