2025-04-04 ミュンヘン大学(LMU)
<関連情報>
- https://www.lmu.de/en/newsroom/news-overview/news/nanotechnology-dna-origami-boosts-sensitivity-of-rapid-tests.html
- https://www.nature.com/articles/s41467-025-57385-6
ラテラルフロー免疫測定におけるDNA折り紙によるシグナル増幅 DNA origami signal amplification in lateral flow immunoassays
Heini Ijäs,Julian Trommler,Linh Nguyen,Stefan van Rest,Philipp C. Nickels,Tim Liedl & Maximilian J. Urban
Nature Communications Published:04 April 2025
DOI:https://doi.org/10.1038/s41467-025-57385-6
Abstract
Lateral flow immunoassays (LFIAs) enable a rapid detection of analytes in a simple, paper-based test format. Despite their multiple advantages, such as low cost and ease of use, their low sensitivity compared to laboratory-based testing limits their use in e.g. many critical point-of-care applications. Here, we present a DNA origami-based signal amplification technology for LFIAs. DNA origami is used as a molecularly precise adapter to connect detection antibodies to tailored numbers of signal-generating labels. As a proof of concept, we apply the DNA origami signal amplification in a sandwich-based LFIA for the detection of cardiac troponin I (cTnI) in human serum. We show a 55-fold improvement of the assay sensitivity with 40-nm gold nanoparticle labels and an adjustable signal amplification of up to 125-fold with fluorescent dyes. The technology is compatible with a wide range of existing analytes, labels, and sample matrices, and presents a modular approach for improving the sensitivity and reliability of lateral flow testing.
