2025-07-11 中国科学院(CAS)
<関連情報>
- https://english.cas.cn/head/202507/t20250710_1047139.shtml
- https://www.cell.com/cell/fulltext/S0092-8674(25)00673-7
全マウス末梢神経のサブセル分解能での高速マッピング High-speed mapping of whole-mouse peripheral nerves at subcellular resolution
Mei-Yu Sh ∙ Yuchen Yao, ∙ Miao Wang ∙ … ∙ Pak-Ming Lau ∙ Cheng Xu ∙ Guo-Qiang Bi
Cell Accepted June: 5, 2025
DOI:https://doi.org/10.1016/j.cell.2025.06.011
Graphical abstract
Highlights
- Imaging of whole adult mouse at uniform subcellular resolution within 40 h
- Reconstruction of 191 Thy1-EGFP spinal neurons and cross-segment projections
- Mapping of immunolabeled sympathetic projections and their perivascular patterns
- Resolving virus-labeled architecture of vagus nerves and complex single-fiber routes
Summary
In contrast to the rapid advancements in mesoscale connectomic mapping of the mammalian brain, similar mapping of the peripheral nervous system has remained challenging due to the body size and complexity. Here, we present a high-speed blockface volumetric imaging system with an optimized workflow of whole-body clearing, capable of imaging the entire adult mouse at micrometer resolution within 40 h. Three-dimensional reconstruction of individual spinal fibers in Thy1-EGFP mice reveals distinct morphological features of sensory and motor projections along the ventral and dorsal rami. Immunostaining facilitates body-wide mapping of sympathetic nerves and their branches, highlighting their perivascular patterns in limb muscles, bones, and most visceral organs. Viral tracing elucidates the fine architecture of vagus nerves and individual vagal fibers, revealing unexpected projection routes to various organs. Our approach offers an effective means to achieve a holistic understanding of cellular-level interactions among different systems that underlie body physiology and disease.
