2025-07-21 ノースカロライナ州立大学(NC State)
<関連情報>
- https://news.ncsu.edu/2025/07/probiotics-can-help-or-hinder-gut-recovery-after-antibiotic-treatment/
- https://journals.asm.org/doi/10.1128/mbio.01468-25
2種のプロバイオティック乳酸菌株によるClostridioides difficileに対する抗生物質投与後のコロニー形成抵抗性の調節の違い Differential modulation of post-antibiotic colonization resistance to Clostridioides difficile by two probiotic Lactobacillus strains
Matthew H. Foley, Arthur S. McMillan, Sarah O’Flaherty, Rajani Thanissery, Molly E. Vanhoy, Mary Gracen Fuller, Rodolphe Barrangou , Casey M. Theriot
mBio Published:21 July 2025
DOI:https://doi.org/10.1128/mbio.01468-25
ABSTRACT
Probiotics are often consumed after antibiotic treatment to prevent antibiotic-associated diarrheal disease, most commonly caused by Clostridioides difficile. However, the impact of probiotic bacteria on the post-antibiotic gut microbiota is undetermined and often overlooked. Here, we examined the effect of a single dose of probiotic Lactobacillus acidophilus NCFM and Lactobacillus gasseri Lg-36 on colonization resistance against C. difficile in an antibiotic-treated mouse model. We found that L. acidophilus administration increased C. difficile infection and impaired the restoration of colonization resistance. In contrast, L. gasseri decreased C. difficile and promoted the return of colonization resistance, presumably through a putative bacteriocin inhibiting C. difficile. However, L. gasseri transiently colonizes the mouse gut, and its administration impacts colonization resistance after it is no longer detectable. We analyzed the gut microbiota of mice and found that members of the understudied Muribaculaceae family were enriched after L. gasseri administration and associated with colonization resistance. Using Muribaculum intestinale and Duncaniella muris, we determined that elevated growth of these species can restrict C. difficile growth in vitro, suggesting that these bacteria may play a role in establishing colonization resistance in vivo. These findings highlight the potential pitfalls of specific probiotic strains taken after antibiotic treatment and support the need for further investigations of their influence on the gut microbiota post-antibiotic. Additionally, this work supports the role of the Muribaculaceae as beneficial gut commensals that can contribute to colonization resistance against C. difficile and illustrates the need to decipher community interactions in complex microbial consortia.
