2025-09-29 国立循環器病研究センター,九州大学,国立精神・神経医療研究センター
<関連情報>
- https://www.ncvc.go.jp/pr/release/pr_49318/
- https://www.cell.com/cell-reports/fulltext/S2211-1247(25)01017-4
AVID mouse: A versatile platform for real-time, multiscale ATP imaging and spatial systems metabolism analysis in living mice
Yuichiro Ohnishi ∙ Daiki Setoyama ∙ Hideki Miwa ∙ … ∙ Motoko Yanagita ∙ Hiromi Imamura ∙ Masamichi Yamamoto
Cell Reports Published:September 12, 2025
DOI:https://doi.org/10.1016/j.celrep.2025.116246
Highlights
- The AVID mouse enables real-time, multiscale ATP imaging in living mice
- Quantitative ATP dynamics were captured across organs and cellular compartments
- Organ-specific responses to physiological and pathological stimuli were visualized
- AVID provides a versatile platform to study spatially resolved energy metabolism in vivo
Summary
We developed the AVID (ATP visualization in vivo directly) mouse, a genetically encoded biosensor mouse enabling real-time, multiscale imaging of ATP dynamics across the whole body, organs, and cellular compartments in living animals. AVID revealed previously undetectable localized ATP depletion near the central vein of the liver after myocardial infarction, spatially linked to kynurenic acid accumulation—a phenomenon invisible to conventional bulk metabolomics. By seamlessly integrating macroscopic organ-level imaging with microscopic spatial metabolomics, AVID establishes a new framework for spatial systems metabolism. Beyond myocardial infarction, this platform offers broad applicability to study organ-organ metabolic communication, spatial metabolic heterogeneity, and localized metabolic shifts across diverse physiological and pathological contexts, providing a transformative resource for metabolic research.
