2025-11-11 新潟大学
Web要約 の発言:
(A) 1細胞シナプトームマッピング法のワークフロー。
(B)マウス大脳皮質ニューロンにおける強化シナプスとサイレントシナプスの1細胞内分布への応用。PSD95陽性シナプス(左)における細胞表面GluA2密度の1細胞シナプトームを取得し(中央)、強化シナプス(赤紫)とサイレントシナプス(水色)をマッピングした(右)。
<関連情報>
- https://www.bri.niigata-u.ac.jp/research/result/002372.html
- https://www.bri.niigata-u.ac.jp/research/result/20251111result.pdf
- https://www.nature.com/articles/s41467-025-65813-w
哺乳類脳における内因性タンパク質サブポピュレーションの単一細胞シナプトームマッピング Single-cell synaptome mapping of endogenous protein subpopulations in mammalian brain
Motokazu Uchigashima,Risa Iguchi,Kazuma Fujii,Kaito Shiku,Pratik Kumar,Xinyi Liu,Mari Isogai,Chiaki Hoshino,Manabu Abe,Motohiro Nozumi,Yosuke Okamura,Michihiro Igarashi,Kenji Sakimura,Ryoma Bise,Luke D. Lavis & Takayasu Mikuni
Nature Communications Published:11 November 2025
DOI:https://doi.org/10.1038/s41467-025-65813-w
Abstract
Different spatial or temporal protein populations, such as cell-surface/intracellular or pre-existing/nascent subpopulations, determine the basal and activity-induced functions of individual synapses within a neuron in vivo. Here, we developed a simple and generalizable platform to image different spatial and temporal subpopulations of endogenous proteins at thousands of synapses in single neurons in the mammalian brain. The platform is based on the development, improvement and integration of CRISPR-Cas9-mediated protein labeling methods, chemical tag labeling techniques, and a semi-automatic analytical pipeline. The combined platform enables whole-cell mapping of total, cell-surface, intracellular, pre-existing, nascent or nascent-and-surface populations of endogenous proteins, such as receptor, scaffold and signaling proteins, at thousands of synapses in individual neurons in living or fixed mouse brain. Our single-cell “synaptome” mapping of endogenous protein subpopulations comprehensively visualizes the spatial representation of synapse diversity in protein localization, trafficking and turnover, providing valuable insights into single-cell organization and computations in the brain.
