2025-12-17 九州大学
図:sci-mtChIL-seqを用いた遺伝子制御機構の解析方法
<関連情報>
- https://www.kyushu-u.ac.jp/ja/researches/view/1381
- https://www.kyushu-u.ac.jp/f/64175/25_1217_01.pdf
- https://www.nature.com/articles/s41467-025-67016-9
単一細胞マルチエピゲノムデータ統合フレームワークによるエピゲノムダイナミクスの再構築 Reconstructing epigenomic dynamics through a single-cell multi-epigenome data integration framework
Takeru Fujii,Kosuke Tomimatsu,Michiko Kato,Miho Ito,Shoko Sato,Hitoshi Kurumizaka,Yuko Sato,Kazumitsu Maehara,Hiroshi Kimura,Akihito Harada & Yasuyuki Ohkawa
Nature Communications Published:17 December 2025
DOI:https://doi.org/10.1038/s41467-025-67016-9
Abstract
Transcriptional regulation arises from the dynamic and combinatorial actions of multiple regulatory factors on genomic DNA. Although many epigenomic regulators have been identified, the precise order in which these factors accumulate at individual gene loci to activate transcription remains unclear. Here we show a single-cell data integration framework that infers the binding order of multiple chromatin factors at single-cell resolution. Central to this framework is sci-mtChIL-seq, a scalable single-cell method that simultaneously profiles genome-wide binding of RNA polymerase II (RNAPII) and diverse epigenomic regulators. By defining transcriptional states through RNAPII occupancy and integrating multiple sci-mtChIL-seq datasets, we systematically link the combinatorial patterns of transcription factor binding, histone modifications and chromatin remodeling. This framework reveals the temporal coordination among chromatin factors during transcriptional activation, providing a powerful approach to uncover context-dependent epigenomic dynamics and the principles of gene regulation in complex cellular systems.
