2024-08-05 イリノイ大学アーバナ・シャンペーン校
<関連情報>
- https://aces.illinois.edu/news/honey-added-yogurt-supports-probiotic-cultures-digestive-health
- https://www.sciencedirect.com/science/article/pii/S0022316624003328
- https://www.sciencedirect.com/science/article/pii/S0022316624000282
ビフィズス菌アニマリス亜種ラクティスDN-173 010/CNCM I-2494を添加したヨーグルトに蜂蜜を加えるとプロバイオティクスの濃縮をサポートするが、健康な成人の腸管通過時間は短縮しない: 無作為対照クロスオーバー試験 Honey Added to Yogurt with Bifidobacterium animalis subsp. lactis DN-173 010/CNCM I-2494 Supports Probiotic Enrichment but Does Not Reduce Intestinal Transit Time in Healthy Adults: A Randomized, Controlled, Crossover Trial
Annemarie R Mysonhimer, Marina D Brown, David A Alvarado, Eva Cornman, Myra Esmail, Tehila Abdiel, Karen Gutierrez, Jorge Vasquez, Corinne N Cannavale, Michael J Miller, Naiman A Khan, Hannah D Holscher
The Journal of Nutrition Available online: 1 June 2024
DOI:https://doi.org/10.1016/j.tjnut.2024.05.028
Abstract
Background
Honey improves probiotic survival in vitro. However, if this effect translates to humans has not been investigated.
Objectives
We aimed to determine effects of honey plus yogurt containing the probiotic Bifidobacterium animalis subsp. lactis DN-173 010/CNCM I-2494 (B. animalis) on intestinal transit time, probiotic enrichment, digestive health, mood, and cognition in adults.
Methods
Sixty-six healthy adults (34 female; 33.6 ± 9.8 y; 24.6 ± 3.0 kg/m2) in a crossover trial were randomly assigned to 2-wk yogurt conditions in a counterbalanced order with ≥4-wk washout: 1) Honey (HON): yogurt plus honey and 2) Negative Control (NC): heat-treated yogurt plus sugar. Of the participants, n = 62 completed the trial, and n = 37 (17 female; 32.0 ± 8.3 y; 25.0 ± 2.9 kg/m2) elected to enroll in a third condition (a nonrandomized study extension) after ≥4-wk washout with a reference Positive Control (PC): yogurt plus sugar. At baseline and end of each of the 3 conditions, intestinal transit time was measured with dye capsules; probiotic abundance with fecal DNA 16S sequencing; digestive health with symptom/function records, Bristol stool consistency, Gastrointestinal Tolerability, and Gastrointestinal Quality of Life Index; mood with Positive and Negative Affect Schedule-Short Form, Depression Anxiety Stress Scales-42, Patient-Reported Outcomes Measurement Information System questionnaires, and an emotional image task; and cognition with a spatial reconstruction task. Data were analyzed using linear mixed-effects models (LMMs) with significance at P ≤ 0.05. Baseline and end data were included in the LMM, with fixed effects being treatment, time, treatment by time interaction, and baseline covariate, and the random effect being the participant.
Results
B. animalis was enriched in HON (d = 3.54; P = 0.0002) compared to controls with linear discriminant analysis effect size. Intestinal transit time, gastrointestinal health, mood, and cognition did not differ between conditions (LMM: Ps > 0.05).
Conclusions
Yogurt + honey enriched B. animalis but did not reduce intestinal transit time or have other functional gastrointestinal, mood, or cognitive effects in adults.
This trial was registered at www.clinicaltrials.gov as NCT04187950 and NCT04901390.
ハチミツの品種がヨーグルト中のBifidobacterium animalis ssp. lactisの生存率に及ぼす影響の違いを試験管内消化シミュレーションにより明らかにした Honey Varietals Differentially Impact Bifidobacterium animalis ssp. lactis Survivability in Yogurt through Simulated In Vitro Digestion
David A Alvarado, Luis Alberto Ibarra-Sánchez, Annemarie R Mysonhimer, Tauseef A Khan, Rong Cao, Michael J Miller, Hannah D Holscher
The Journal of Nutrition Available online: 12 January 2024
DOI:https://doi.org/10.1016/j.tjnut.2024.01.010
Abstract
Background
Bifidobacterium animalis ssp. lactis DN-173 010/CNCM I-2494 (B. animalis) is a probiotic strain commonly added to yogurt. Yogurt and honey are a popular culinary pairing. Honey improves bifidobacteria survival in vitro. However, probiotic survival in yogurt with honey during in vitro digestion has not been investigated.
Objectives
The study aimed to evaluate the effects of different honey varietals and concentrations on B. animalis survivability in yogurt through in vitro digestion.
Methods
Yogurt with honey or control-treated samples underwent in vitro simulated oral, gastric, and intestinal digestion. B. animalis cells were enumerated on de Man Rogosa and Sharpe (MRS) medium followed by an overlay with a modified selective MRS medium; all underwent anaerobic incubation. B. animalis were enumerated predigestion and after oral, gastric, and intestinal digestion. There were 2 study phases: Phase 1 tested 4 honey varietals at 20% wt/wt per 170 g yogurt, and Phase 2 tested 7 dosages of clover honey (20, 14, 10, 9, 8, 6, and 4% wt/wt) per 170 g yogurt.
Results
Similar B. animalis counts were observed between all treatments after oral and gastric digestion (<1 Log colony forming units (CFU)/g probiotic reduction). Higher B. animalis survivability was observed in yogurt with clover honey after exposure to simulated intestinal fluids (∼3.5 Log CFU/g reduction; P < 0.05) compared to all control treatments (∼5.5 Log CFU/g reduction; P < 0.05). Yogurt with 10–20% wt/wt clover honey increased B. animalis survivability after simulated in vitro digestion (≤ ∼4.7 Log CFU/g survival; P < 0.05).
Conclusions
Yogurt with added honey improves probiotic survivability during in vitro digestion. The effective dose of clover honey in yogurt was 10–20% wt/wt per serving (1–2 tablespoons per 170 g yogurt) for increased probiotic survivability during in vitro digestion.
