2025-01-20 ミュンヘン大学(LMU)
<関連情報>
- https://www.lmu.de/en/newsroom/news-overview/news/transposable-elements-ancient-viral-dna-shapes-early-embryo-development.html
- https://www.cell.com/cell/fulltext/S0092-8674(24)01426-0
転写開始のアトラスは、哺乳類の初期発生における遺伝子と転移因子の発現の制御原理を明らかにする An atlas of transcription initiation reveals regulatory principles of gene and transposable element expression in early mammalian development
Marlies E. Oomen∙ Diego Rodriguez-Terrones∙ Mayuko Kurome∙ … ∙ Eckhard Wolf∙ Henrik Kaessmann∙ Maria-Elena Torres-Padilla
Cell Published:January 20, 2025
DOI:https://doi.org/10.1016/j.cell.2024.12.013
Graphical abstract
Highlights
•Mapping transcription start site reveals genome regulatory principles in early embryos
•Transposable elements exhibit convergent and divergent patterns across species
•LTRs, SINEs, LINEs, and DNA transposons drive chimeric transcripts in early embryos
•Ancient transposable elements are transcribed during mammalian development
Summary
Transcriptional activation of the embryonic genome (EGA) is a major developmental landmark enabling the embryo to become independent from maternal control. The magnitude and control of transcriptional reprogramming during this event across mammals remains poorly understood. Here, we developed Smart-seq+5′ for high sensitivity, full-length transcript coverage and simultaneous capture of 5′ transcript information from single cells and single embryos. Using Smart-seq+5′, we profiled 34 developmental stages in 5 mammalian species and provide an extensive characterization of the transcriptional repertoire of early development before, during, and after EGA. We demonstrate widespread transposable element (TE)-driven transcription across species, including, remarkably, of DNA transposons. We identify 19,657 TE-driven genic transcripts, suggesting extensive TE co-option in early development over evolutionary timescales. TEs display similar expression dynamics across species and species-specific patterns, suggesting shared and divergent regulation. Our work provides a powerful resource for understanding transcriptional regulation of mammalian development.
