2025-05-12 東京科学大学
図1. Localizatome(ローカラゼトーム)データベース概要
<関連情報>
- https://www.isct.ac.jp/ja/news/d4jwy7o76ojx
- https://www.isct.ac.jp/plugins/cms/component_download_file.php?type=2&pageId=&contentsId=1&contentsDataId=1526&prevId=&key=a2a28b2e6079a2b009302c84c409cb25.pdf
- https://academic.oup.com/database/article/doi/10.1093/database/baaf028/8117019
タンパク質におけるストレス依存的な細胞内局在変化のデータベースLocalizatome Localizatome: a database for stress-dependent subcellular localization changes in proteins
Takahide Matsushima , Yuki Naito , Tomoki Chiba , Ryota Kurimoto , Keiko Itano , Koji Ochiai , Koichi Takahashi , Naoki Goshima , Hiroshi Asahara
Database Published:21 April 2025
DOI:https://doi.org/10.1093/database/baaf028
Abstract
Understanding protein subcellular localization and its dynamic changes is crucial for elucidating cellular function and disease mechanisms, particularly under stress conditions, where protein localization changes can modulate cellular responses. Currently available databases provide insights into protein localization under steady-state conditions; however, stress-related dynamic localization changes remain poorly understood. Here, we present the Localizatome, a comprehensive database that captures stress-induced protein localization dynamics in living cells. Using an original high-throughput microscopy system and machine learning algorithms, we analysed the localization patterns of 10 287 fluorescent protein-fused human proteins in HeLa cells before and after exposure to oxidative stress. Our analysis revealed that 1910 proteins exhibited oxidative stress-dependent localization changes, particularly forming distinct foci. Among them, there were stress granule assembly factors and autophagy-related proteins, as well as components of various signalling pathways. Subsequent characterization identified some specific amino acid motifs and intrinsically disordered regions associated with stress-induced protein redistribution. The Localizatome provides open access to these data through a web-based interface, supporting a wide range of studies on cellular stress response and disease mechanisms.
