2025-11-20 理化学研究所,実中研
3種のエピゲノム制御によるマーモセット体細胞クローン胚の品質改善とES細胞樹立
<関連情報>
- https://www.riken.jp/press/2025/20251120_2/index.html
- https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(25)00314-5
コモンマーモセットにおける改良型体細胞核移植を用いたクローン胚盤胞からの胚性幹細胞の誘導 Derivation of embryonic stem cells from cloned blastocysts using improved somatic cell nuclear transfer in common marmosets
Shogo Matoba ∙ Yoko Kurotak ∙ Satoshi Funaya ∙ … ∙ Yuichiro Higuchi ∙ Erika Sasaki ∙ Atsuo Ogura
Stem Cell Reports Published:November 13, 2025
DOI:https://doi.org/10.1016/j.stemcr.2025.102710
Highlights
- First derivation of ntESCs from cloned marmoset embryos with epigenetic treatment
- Triple treatment with TSA, Kdm4d, and G9a inhibitor improves blastocyst development
- Established ntESCs exhibit pluripotency, germ layer differentiation, and genomic integrity
- Transcriptome analysis reveals partial reprogramming defects in translation-associated genes
Summary
The common marmoset (Callithrix jacchus) is a genetically modifiable non-human primate increasingly used in biomedical research. Here, we established a method for deriving embryonic stem cells (ESCs) from blastocysts generated by somatic cell nuclear transfer (SCNT) in the marmoset. Injection of histone demethylase Kdm4d mRNA enabled efficient reprogramming of somatic nuclei, allowing blastocyst formation in 14.5% from fibroblasts. Combining this method with a G9a/EHMT2 histone methyltransferase inhibitor improved blastocyst quality and allowed derivation of nuclear transfer ESCs (ntESCs), including wild-type and GFP-transgenic lines. These ntESCs exhibited normal karyotypes and pluripotency. Nuclear and mitochondrial DNA analyses confirmed their nuclear donor origin and cytoplasmic inheritance from recipient oocytes. Transcriptome analysis identified abnormally expressed genes in ntESCs present in a line-dependent and independent manner, suggesting partial reprogramming resistance. Our study establishes a marmoset SCNT method enabling derivation of ntESCs and provides a new platform for preserving and engineering marmoset genetic resources.
