2024-01-23 米国国立標準技術研究所(NIST)
◆この発見は、多くの薬物治療のデザインに影響を及ぼす可能性があります。細胞膜が生成する電場が主に中性で帯電していないナノ粒子を反発させる原因であることが確認され、これはタンパク質などの薬物治療の効果に寄与する可能性があります。この発見は、ナノスケールでの分子の相互作用の理解を深め、薬物の設計や治療に新しい視点を提供します。
<関連情報>
- https://www.nist.gov/news-events/news/2024/01/cells-electric-fields-keep-nanoparticles-bay-scientists-confirm
- https://pubs.acs.org/doi/full/10.1021/jacs.3c12348
電荷を帯びた生体膜は、表面誘電泳動と対イオン圧によって大きな中性分子をはじく Charged Biological Membranes Repel Large Neutral Molecules by Surface Dielectrophoresis and Counterion Pressure
Marcel Aguilella-Arzo, David P. Hoogerheide, Mathieu Doucet, Hanyu Wang, and Vicente M. Aguilella
Journal of the American Chemical Society Published:January 16, 2024
DOI:https://doi.org/10.1021/jacs.3c12348
Abstract
Macromolecular crowding is the usual condition of cells. The implications of the crowded cellular environment for protein stability and folding, protein–protein interactions, and intracellular transport drive a growing interest in quantifying the effects of crowding. While the properties of crowded solutions have been extensively studied, less attention has been paid to the interaction of crowders with the cellular boundaries, i.e., membranes. However, membranes are key components of cells and most subcellular organelles, playing a central role in regulating protein channel and receptor functions by recruiting and binding charged and neutral solutes. While membrane interactions with charged solutes are dominated by electrostatic forces, here we show that significant charge-induced forces also exist between membranes and neutral solutes. Using neutron reflectometry measurements and molecular dynamics simulations of poly(ethylene glycol) (PEG) polymers of different molecular weights near charged and neutral membranes, we demonstrate the roles of surface dielectrophoresis and counterion pressure in repelling PEG from charged membrane surfaces. The resulting depletion zone is expected to have consequences for drug design and delivery, the activity of proteins near membrane surfaces, and the transport of small molecules along the membrane surface.
