2024-06-19 リンショーピング大学
<関連情報>
- https://liu.se/en/news-item/effektivare-cancerbehandling-med-jontronisk-pump
- https://www.sciencedirect.com/science/article/pii/S0168365924001974
連続的なイオントロニック化学療法は、鳥類のin vivo胚モデルにおいて脳腫瘍の成長を減少させる Continuous iontronic chemotherapy reduces brain tumor growth in embryonic avian in vivo models
Verena Handl, Linda Waldherr, Theresia Arbring Sjöström, Tobias Abrahamsson, Maria Seitanidou, Sabine Erschen, Astrid Gorischek, Iwona Bernacka-Wojcik, Helena Saarela, Tamara Tomin, Sophie Elisabeth Honeder, Joachim Distl, Waltraud Huber, Martin Asslaber, Ruth Birner-Grünberger, Ute Schäfer, Magnus Berggren, Rainer Schindl, Silke Patz, Daniel T. Simon, Nassim Ghaffari-Tabrizi-Wizsy
Journal of Controlled Release Available online: 11 April 2024
DOI:https://doi.org/10.1016/j.jconrel.2024.03.044
Highlights
- GemIPs deliver the chemotherapeutic gemcitabine to continously hold local concentration profiles within a therapeutically relevant range.
- GemIP treatment of brain tumors grown on an avian host model using GemIPs induces apoptotic events and cell cycle arrest.
- GemIP treatment interferes with tumor growth and vascularization and outperforms conventional metronomic therapies.
- Iontronics enable on-site and on-demand administration of so far unconsidered chemotherapeutics for brain tumors.
Abstract
Local and long-lasting administration of potent chemotherapeutics is a promising therapeutic intervention to increase the efficiency of chemotherapy of hard-to-treat tumors such as the most lethal brain tumors, glioblastomas (GBM). However, despite high toxicity for GBM cells, potent chemotherapeutics such as gemcitabine (Gem) cannot be widely implemented as they do not efficiently cross the blood brain barrier (BBB). As an alternative method for continuous administration of Gem, we here operate freestanding iontronic pumps – “GemIPs” – equipped with a custom-synthesized ion exchange membrane (IEM) to treat a GBM tumor in an avian embryonic in vivo system. We compare GemIP treatment effects with a topical metronomic treatment and observe that a remarkable growth inhibition was only achieved with steady dosing via GemIPs. Daily topical drug administration (at the maximum dosage that was not lethal for the embryonic host organism) did not decrease tumor sizes, while both treatment regimes caused S-phase cell cycle arrest and apoptosis. We hypothesize that the pharmacodynamic effects generate different intratumoral drug concentration profiles for each technique, which causes this difference in outcome. We created a digital model of the experiment, which proposes a fast decay in the local drug concentration for the topical daily treatment, but a long-lasting high local concentration of Gem close to the tumor area with GemIPs. Continuous chemotherapy with iontronic devices opens new possibilities in cancer treatment: the long-lasting and highly local dosing of clinically available, potent chemotherapeutics to greatly enhance treatment efficiency without systemic side-effects.
Significance statement
Iontronic pumps (GemIPs) provide continuous and localized administration of the chemotherapeutic gemcitabine (Gem) for treating glioblastoma in vivo. By generating high and constant drug concentrations near the vascularized growing tumor, GemIPs offer an efficient and less harmful alternative to systemic administration. Continuous GemIP dosing resulted in remarkable growth inhibition, superior to daily topical Gem application at higher doses. Our digital modelling shows the advantages of iontronic chemotherapy in overcoming limitations of burst release and transient concentration profiles, and providing precise control over dosing profiles and local distribution. This technology holds promise for future implants, could revolutionize treatment strategies, and offers a new platform for studying the influence of timing and dosing dependencies of already-established drugs in the fight against hard-to-treat tumors.
Graphical abstract
