亜塩素酸水を活用した乳牛の皮膚消毒技術を実証～消毒作業の効率化を目指して～

2025-03-04

2025-03-04 北海道大学

北海道大学大学院獣医学研究院の市居修教授らの研究グループは、乳牛の手術前消毒において、亜塩素酸水を用いた新たな皮膚消毒法を開発し、従来法と同等の消毒効果を確認しました。この方法は、従来の消毒手順よりも工程が少なく、作業時間の短縮が可能であり、動物医療現場での消毒作業の効率化や、動物の拘束時間短縮につながる可能性があります。研究成果は、2025年2月26日に『Frontiers in Veterinary Science』誌に掲載されました。

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乳牛の手術部位消毒への亜塩素酸水の応用
Application of chlorous acid water for disinfection of surgical site in dairy cows

Osamu Ichii,Teppei Nakamura,Masaya Hiraishi,Takashi Namba,Md. Zahir Uddin Rubel,Takuya Umeyama,Megumi Asai
Frontiers in Veterinary Science Published:26 February 2025
DOI:https://doi.org/10.3389/fvets.2025.1444674

Disinfection is crucial for preventing surgical site infections. Recently, the effectiveness of sanitizers using chlorous acid (HClO₂) under conditions rich in organic matter has been reported, and chlorous acid water (CAW) has been approved as a food additive. This study evaluated the potential of CAW as a new presurgical disinfectant for cattle. The experiments were performed on the paralumbar fossa of cattle in Sapporo during March (winter to spring) and August (summer). Colony-forming units (CFUs) of standard plate count bacteria (SPCB), Enterococcus faecalis (EF), Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus spp. (SP) were analyzed as indicators of bacterial load. SPCB and SP were abundantly detected, exceeding 6 log₁₀ CFU/100 g on clipped hair and 6 log₁₀ CFU/100 cm² on the skin immediately after clipping, with no significant seasonal differences. The bacterial load on the skin was evaluated at three time points: after clipping, cleansing, and disinfection. Clipping and cleansing with liquid soap were common procedures, following this, either the standard disinfection protocol using 7.5% iodine scrub for 1 min, 10% povidone-iodine for 5 min, and 70% alcohol for 5 min (SPA), or a modified protocol using CAW with contact times of 15, 10, or 5 min (CAW15, CAW10, CAW5) were performed separately. The cleansing procedure significantly reduced the SPCB, EF, and SP on the skin after clipping, and all disinfection methods significantly decreased the SP after cleansing. Draping significantly enhanced the disinfection efficiency of the SPA, CAW10, and CAW5 protocols. The CAW procedure did not alter skin histology in the paralumbar fossa or udder compared to 10% povidone-iodine or 70% alcohol. Our data suggest that the disinfection method using CAW is useful and comparable to routine disinfection methods and might reduce the time required for presurgical disinfection in farm fields.