2025-05-28 医薬基盤・健康・栄養研究所
図1. 腸内細菌特異的モノクローナル抗体の樹立と迅速診断系への応用
<関連情報>
- https://www.nibn.go.jp/pr/press/20250528.html
- https://www.nibn.go.jp/pr/press/documents/20250528.pdf
- https://www.nature.com/articles/s41598-025-01144-6
様々な診断システムにおける腸型特異的抗体の確立 Establishment of enterotype-specific antibodies for various diagnostic systems
Ken Yoshii,Eri Node,Mari Furuta,Yoko Tojima,Ayu Matsunaga,Jun Adachi,Narimi Takaai,Makiko Morita,Koji Hosomi & Jun Kunisawa
Scientific Reports Published:14 May 2025
DOI:https://doi.org/10.1038/s41598-025-01144-6
Abstract
This study demonstrates that monoclonal antibodies can be developed to targeting specific gut bacteria prevalent in the Japanese population and the potential for creating a novel diagnostic system using these antibodies. In this study, we established specific antibodies against representative bacteria from the genera Bacteroides, Faecalibacterium, and Prevotella and showed that they could be detected using ELISA, flow cytometry, and western blot analysis. Furthermore, a technique to quantify target bacteria was developed by combining these antibodies in a sandwich ELISA, enabling the quantification of bacteria in human fecal samples. This technology serves as a foundational method for rapidly and easily measuring gut bacteria and is expected to evolve into a powerful tool for analyzing the impact of gut bacteria on health, as well as for personalized health management based on individual gut environments.
