2025-08-27 清華大学
Figure 1. Emergence of lineage-specific DNAme in early mammalian development
<関連情報>
- https://www.tsinghua.edu.cn/en/info/1245/14454.htm
- https://www.cell.com/cell-reports/fulltext/S2211-1247(25)00561-3
哺乳類初期発生におけるDNAメチルーム-トランスクリプトーム動態の着床前～着床後移行過程の解明 Dissecting pre- to post-implantation transition of DNA methylome-transcriptome dynamics in early mammalian development
Qingyuan Zhu ∙ Ying Liu ∙ Xin Hao ∙ … ∙ Changqing Zheng ∙ Xianwen Ren ∙ Fan Zhou
Cell Reports Published:June 3, 2025
DOI:https://doi.org/10.1016/j.celrep.2025.115790
Highlights
- Species-conserved lineage-specific DNA methylation remodeling occurs during PPT
- Visceral endoderm subtypes show segregated methylation dynamics
- DNA methylation difference of parental transposons reveals asynchronous erasure
- Identified promoters exhibit non-canonical DNA methylation-transcription coupling
Summary
Pre- to post-implantation transition is an essential step for early mammalian development. The epigenome dynamics such as DNA methylome and transcriptome of embryos have been analyzed, while the coordination between these two molecular layers controlling lineage fate remained elusive. Here, with a multidimensional profiling of peri-implantation embryos, we present the emerging lineage-specific DNA methylation (DNAme) patterns across species. The maternal and paternal DNA methylation levels exhibit differential dynamics in transposon elements. Genes with lineage-specific unmethylated promoters early in development retain this state and are expressed later, suggesting a role in lineage fate determination. By measuring both molecular dimensions simultaneously in individual developing cells, we identified a group of gene promoters with positive correlation between DNAme and RNAex along epiblast development. Functional validation suggested DNAme at these promoters contributes to non-canonical DNAme-RNAex dynamics, potentially via complex TF-mediated or epigenetic regulation. This study provides clues for understanding molecular regulation of peri-implantation embryogenesis.
