2025-09-05 理化学研究所,ポーラ化成工業株式会社
ヒト毛周期の時系列的な解析から見いだした皮膚組織再構築の仕組み
<関連情報>
- https://www.riken.jp/press/2025/20250905_3/index.html
- https://www.cell.com/cell-reports/fulltext/S2211-1247(25)00967-2
ヒト毛周期の単一細胞トランスクリプトーム再構築:皮膚組織リモデリングの時間的動態の捕捉 Single-cell transcriptomic reconstruction of the human hair cycle: Capturing the temporal dynamics of skin tissue remodeling
Jun Yokota ∙ Takamasa Gomi ∙ Yasuko Harada ∙ … ∙ Hiroki Machida ∙ Yuki Shirai ∙ Hironobu Fujiwara
Cell Reports Published:August 28, 2025
DOI:https://doi.org/10.1016/j.celrep.2025.116196
Highlights
- A single-cell dataset reveals hair-cycle-dependent shifts in cellular states and interactions
- Fibroblasts, vessels, and leukocytes contribute to tissue remodeling in regressing follicles
- ECM remodeling, angiogenesis, and phagocytosis proceed sequentially during regression
- Three cell populations signal reciprocally with follicular keratinocytes during regression
Summary
The hair cycle is a valuable model for studying tissue remodeling processes in adult mammals. Time course single-cell transcriptomics offers a powerful approach for characterizing changes in cellular states and interactions; however, its application in human tissues remains challenging. Here, we performed single-cell RNA sequencing on 19 human skin tissues, each containing a single hair follicle. We reconstructed a pseudotemporal progression of the hair cycle—termed the “pseudo-hair cycle”—by computationally ordering samples according to their gene expression profiles. The pseudo-hair cycle delineates the continuum of gene expression changes occurring in 16 distinct epithelial and dermal cell populations throughout the hair cycle, revealing specific cellular states and interactions. We identified hair follicle keratinocytes, dermal sheath fibroblasts, vascular endothelial cells, and leukocytes as key contributors to tissue remodeling during catagen. Our study provides a high-resolution temporal dataset for investigating the dynamic molecular and cellular mechanisms underlying skin remodeling in humans.
