2025-12-23 広島大学
図 ビオチンメチルエステル (BME)による細胞内への迅速なビオチン供給と応用
<関連情報>
- https://www.hiroshima-u.ac.jp/news/94931
- https://www.hiroshima-u.ac.jp/system/files/278473/%E5%A0%B1%E9%81%93%E7%99%BA%E8%A1%A8%E8%B3%87%E6%96%99.pdf
- https://www.nature.com/articles/s42003-025-09176-4
ビオチンメチルエステルはRUSHシステムにおける貨物の放出を促進し、TurboIDによる迅速なビオチン化を可能にする Biotin methyl ester enhances cargo release in RUSH system and enables rapid biotinylation with TurboID
Taisei Uehara,Arata Takiguchi,Takuro Tojima,Akihiko Nakano,Shion Kagawa,Tatsuo Nehira,Takunori Satoh & Akiko K. Satoh
Communications Biology Published:16 December 2025
DOI:https://doi.org/10.1038/s42003-025-09176-4
Abstract
Biotin-streptavidin technology is a key tool in biotechnology. The engineering of streptavidin or streptavidin-like molecules and organic synthesis of biotin derivatives offer a variety of applications to meet the needs of researchers. Here, we report that owing to its masked charged carboxyl group, biotin methyl ester (BME) improves cell penetration and allows the retention using selective hooks (RUSH) system to initiate cargo release promptly and uniformly in all cells. In addition, because of the rapid hydrolysis of BME to biotin inside cells, BME enabled faster biotinylation than biotin, by the promiscuous biotin ligase TurboID. These properties allow BME to act as a biotin prodrug, enhance the RUSH system and TurboID, and highlight the potential of biotin-streptavidin technology for numerous applications in living cells. Additionally, BME may be a good biotin supplement for patients with multivitamin-responsive inherited metabolic disorders.
