2026-03-16 中国科学院（CAS）
＜関連情報＞
- https://english.cas.cn/newsroom/research-news/202603/t20260316_1152735.shtml
- https://pubs.acs.org/doi/10.1021/acs.analchem.5c07520
TOI-IGRA：病原体特異的T細胞応答の分散型定量化のための指先血液バイオセンシング技術 TOI-IGRA: A Fingertip Blood Biosensing Technology for Decentralized Quantification of Pathogen-Specific T-Cell Responses
Yuzhong Xu,Binmao Zhang,Zhen Huang,Mengxing Mou,Tianyuan Zhao,Baozhu Zhang,Hao Yang,Hao Huang,Hai Qu,Wenjuan Wu,Peijin Wang,Qiaohui Zhao,Chunying Dai,Jie Zhang,Liangzhi Xie,Siyan Zhong,Renshuang Cao,Hao Li,Hui Yang,Yujuan Chai,and Xiaotian Tan
Analytical Chemistry Published: March 16, 2026
DOI:https://doi.org/10.1021/acs.analchem.5c07520
Abstract
T-cell immune responses are key components of the adaptive immune system. The Interferon-γ Release Assay (IGRA), a standardized method for evaluating T-cell function, is widely used for tuberculosis (TB) diagnosis. However, conventional IGRA requires venous blood (approximately 5 mL), involves lengthy processing (around 24 h), and relies on burdensome workflows, limiting its applicability in decentralized or resource-limited settings. Here, we present TOI-IGRA, a fingertip blood-based T-cell functional assay built on our in-house Tip Optofluidic Immunoassay (TOI) platform. Requiring only 15–25 μL of capillary blood, TOI-IGRA offers a minimally invasive, rapid, and efficient renovation to the standard IGRA. We demonstrate that fingertip blood yields comparable T-cell functional results with venous blood, and clinical validation in TB patients confirmed diagnostic performance equivalent to that of the standard IGRA. Furthermore, the TOI-IGRA concept can be extended to assess T-cell responses against other pathogens, including SARS-CoV-2. The platform also enables concurrent quantitative analysis of pathogen-specific T-cell responses and binding antibodies from the same fingertip blood sample. With its minimal blood requirement, operational simplicity, and versatility, TOI-IGRA represents a powerful tool for decentralized diagnostics, large-scale immune surveillance, and personalized vaccine monitoring across diverse healthcare settings.
