IBS認知・社会性研究センター、てんかん、筋痙攣、肌のシワなどの治療への貢献が期待される光遺伝学的ツール「Opto-vTrap」を新たに開発
IBS Center for Cognition and Sociality developed a new optogenetic tool, ‘Opto-vTrap’, which is expected to contribute to treatment for epilepsy, muscle spasms, and skin wrinkles
2022-01-10 大韓民国・基礎科学研究院(IBS)
Controlling signal transmission and reception within the brain circuits is necessary for neuroscientists to achieve a better understanding of the brain’s functions. Communication among neuron and glial cells is mediated by various neurotransmitters being released from the vesicles through exocytosis. Thus, regulating vesicular exocytosis can be a possible strategy to control and understand brain circuits.
However, it has been difficult to freely control the activity of brain cells in a spatiotemporal manner using pre-existing techniques. One is an indirect approach that involves artificially controlling the membrane potential of cells, but it comes with problems of changing the acidity of the surrounding environment or causing unwanted misfiring of neurons. Moreover, it is not applicable for use in cells that do not respond to the membrane potential changes, such as glial cells.
To address this problem, South Korean researchers led by Director C. Justin LEE at the Center for Cognition and Sociality within the Institute for Basic Science (IBS) and professor HEO Won Do at Korea Advanced Institute of Science and Technology (KAIST) developed Opto-vTrap, a light-inducible and reversible inhibition system that can temporarily trap vesicles from being released from brain cells. Opto-vTrap directly targets transmitters containing vesicles, and it can be used in various types of brain cells, even the ones that do not respond to membrane potential changes.
In order to directly control the exocytotic vesicles, the research team applied a technology they previously developed in 2014, called light-activated reversible inhibition by assembled trap (LARIAT). This platform can inactivate various types of proteins when illuminated under blue light by instantly trapping the target proteins, like a lariat. Opto-vTrap was developed by applying this LARIAT platform to vesicle exocytosis. When the Opto-vTrap expressing cells or tissues are shined under blue light, the vesicles form clusters and become trapped within the cells, inhibiting the release of transmitters.
Most importantly, the inhibition triggered using this new technique is temporary, which is very important for neuroscience research. Other previous techniques that target vesicle fusion proteins damage them permanently and disable the target neuron for up to 24 hours, which is not appropriate for many behavioral experiments with short time constraints. By comparison, vesicles that were inactivated using Opto-vTrap decluster in about 15 minutes, and the neurons regain their full functions within an hour.
Opto-vTrap directly controls the signal transmitters’ release, enabling the researchers to freely control brain activity. The research team verified the usability of Opto-vTrap in cultured cells and brain tissue slices. Furthermore, they tested the technique in live mice, which enabled them to temporarily remove fear memory from fear-conditioned animals.
In the future, Opto-vTrap will be used to uncover complex interactions between multiple parts of the brain. It will be a highly useful tool for studying how certain brain cell types affect brain function in different circumstances.
Professor Heo stated, “Since Opto-vTrap can be used in various cell types, it is expected to be helpful in various fields of brain science research,” He explained, “We plan to conduct a study to figure out the spatiotemporal brain functions in various brain cell types in a specific environment using Opto-vTrap technology.”
“The usability of Opto-vTrap can extend not only to neuroscience but also to our lives,” explains Director Lee. He added, “Opto-vTrap will contribute not only to elucidate brain circuit mapping but also epilepsy treatment, muscle spasm treatment, and skin tissue expansion technologies.”
This research was published on November 30 in the journal Neuron (IF:17.173).
Learn more about this research at
Schematic diagram of Opto-vTrap, an optogenetic trap for reversible inhibition of vesicular release, synaptic transmission, and behavior
(top left) The components of Opto-vTrap were expressed in the vesicles and cytoplasm. (top right) Shining blue light causes a vesicle clusterization and complete inhibition of exocytosis. After the blue light is turned off, the vesicles decluster and quickly return back to their normal state. It is proposed that Opto-vTrap can serve as a next-generation optogenetic silencer to control brain activity and behavior with minimal confounding effects.
Activation of Opto-vTrap inhibits evoked EPSC in Schaffer-collateral synapses
After Opto-vTrap virus injection, evoked excitatory postsynaptic current (EPSC) was measured in mouse brain slices to test whether activation of Opto-vTrap can selectively inhibit synaptic transmission. In the control mouse, no changes in evoked EPSC amplitude before, light on, and after light on conditions were observed. In Opto-vTrap injected brain slices, evoked EPSC amplitude decreased during light on, which promptly recovered after light off.
Activation of Opto-vTrap inhibits neocortical astrocytic vesicular gliotransmitter
After Opto-vTrap virus injection, glial induced fast inward current (gFIC) was measured in mouse brain slices to test whether Opto-vTrap can selectively inhibit vesicular release in non-excitable cells such as astrocytes. In the control mouse, it was observed that frequency of gFIC is enhanced after TFLLR treatment. In Opto-vTrap injected brain slices, it was observed that TFLLR-induced increase in the frequency of gFICs was completed prevented by Opto-vTrap activation, which promptly recovered after light off.
Activation of Opto-vTrap reversibly inhibits contextual fear memory retrieval.
On day 1, the mouse was exposed to electrical stimulation to induce contextual fear conditioning. On days 2 and 3, the mouse was re-exposed to the same context as on day 1. If the mouse recalls the fear memory, it will remain frozen in the place. In contrast, if the mouse cannot recall the fear memory, they will move around normally in the presence of context. During Opto-vTrap activation experiment in trial 2 on day 2, it was observed that freezing behavior decreased, which is reversed when the light was turned off.
Notes for editors
– References
Joungha Won, Yuriy Pankratov, Minwoo Wendy Jang, Sunpil Kim, Yeonha Ju, Sangkyu Lee, Seung Eun Lee, Arie Kim, Soowon Park, C. Justin Lee* and Won Do Heo*. Opto-vTrap, an optogenetic trap for reversible inhibition of vesicular release, synaptic transmission, and behavior. Neuron. DOI: 10.1016/j.neuron.2021.11.003
– Media Contact
For further information or to request media assistance, please contact C. Justin Lee, Center for Cognition and Sociality, Institute for Basic Science (IBS) (cjl@ibs.re.kr) or William I. Suh at the IBS Communications Team (willisuh@ibs.re.kr)
– About the Institute for Basic Science (IBS)
IBS was founded in 2011 by the government of the Republic of Korea with the sole purpose of driving forward the development of basic science in South Korea. IBS has 30 research centers as of August 2021. There are ten physics, three mathematics, six chemistry, five life science, one Earth science, and five interdisciplinary research centers.
