ペチュニアの花から生まれた、進化したチェリーフレーバー(New and improved cherry flavor courtesy of the petunia flower)

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2022-03-21 パデュー大学
パデュー大学農学部生化学特別教授のNatalia Dudarevaと博士研究員のXing-Qi Huangは、ペチュニアを使って貴重な香料化合物であるベンズアルデヒドの分子レシピを発見しています。この化合物は、ペチュニアだけでなく、多くの果物の香りに含まれている。

ペチュニアの花から生まれた、進化したチェリーフレーバー(New and improved cherry flavor courtesy of the petunia flower)
(Purdue University photo/Xing-Qi Huang)
<関連情報>

植物におけるペルオキシソームのヘテロ二量体酵素がベンズアルデヒド合成に関与すること A peroxisomal heterodimeric enzyme is involved in benzaldehyde synthesis in plants

Xing-Qi Huang,Renqiuguo Li,Jianxin Fu &Natalia Dudareva

Published: 

figure 1

Abstract

Benzaldehyde, the simplest aromatic aldehyde, is one of the most wide-spread volatiles that serves as a pollinator attractant, flavor, and antifungal compound. However, the enzyme responsible for its formation in plants remains unknown. Using a combination of in vivo stable isotope labeling, classical biochemical, proteomics and genetic approaches, we show that in petunia benzaldehyde is synthesized via the β-oxidative pathway in peroxisomes by a heterodimeric enzyme consisting of α and β subunits, which belong to the NAD(P)-binding Rossmann-fold superfamily. Both subunits are alone catalytically inactive but, when mixed in equal amounts, form an active enzyme, which exhibits strict substrate specificity towards benzoyl-CoA and uses NADPH as a cofactor. Alpha subunits can form functional heterodimers with phylogenetically distant β subunits, but not all β subunits partner with α subunits, at least in Arabidopsis. Analysis of spatial, developmental and rhythmic expression of genes encoding α and β subunits revealed that expression of the gene for the α subunit likely plays a key role in regulating benzaldehyde biosynthesis.

有機化学・薬学
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