画期的な顕微鏡技術が細胞内物質輸送の秘密を解き明かす Revolutionary microscopy technique unlocks secrets of intracellular cargo transport
2023-11-16 韓国基礎科学研究院(IBS)
◆この顕微鏡は、蛍光イメージングとiSCAT顕微鏡を統合したデュアルモダリティシステムを備え、特定のラベルが付いたカーゴと付いていないカーゴを同時に観察できます。将来的には、ウイルス感染の調査や抗ウイルスワクチンや薬の効果のリアルタイムモニタリングなど、広範な応用が期待されます。
<関連情報>
- https://www.ibs.re.kr/cop/bbs/BBSMSTR_000000000738/selectBoardArticle.do
- https://www.nature.com/articles/s41467-023-42347-7
細胞内物質輸送の秘密を解き明かす画期的な顕微鏡技術を開発 Long-term cargo tracking reveals intricate trafficking through active cytoskeletal networks in the crowded cellular environment
Jin-Sung Park,Il-Buem Lee,Hyeon-Min Moon,Seok-Cheol Hong & Minhaeng Cho
Nature Communications Published:14 November 2023
DOI:https://doi.org/10.1038/s41467-023-42347-7
Abstract
A eukaryotic cell is a microscopic world within which efficient material transport is essential. Yet, how a cell manages to deliver cellular cargos efficiently in a crowded environment remains poorly understood. Here, we used interferometric scattering microscopy to track unlabeled cargos in directional motion in a massively parallel fashion. Our label-free, cargo-tracing method revealed not only the dynamics of cargo transportation but also the fine architecture of the actively used cytoskeletal highways and the long-term evolution of the associated traffic at sub-diffraction resolution. Cargos frequently run into a blocked road or experience a traffic jam. Still, they have effective strategies to circumvent those problems: opting for an alternative mode of transport and moving together in tandem or migrating collectively. All taken together, a cell is an incredibly complex and busy space where the principle and practice of transportation intriguingly parallel those of our macroscopic world.
