2024-09-09 米国国立標準技術研究所(NIST)
<関連情報>
- https://www.nist.gov/news-events/news/2024/09/now-live-living-cells-can-be-seen-infrared-light
- https://pubs.acs.org/doi/10.1021/acs.analchem.4c02108
生細胞のベンチトップIRイメージング: 単一細胞内の生体分子の全質量をモニターする Benchtop IR Imaging of Live Cells: Monitoring the Total Mass of Biomolecules in Single Cells
Yow-Ren Chang,Seong-Min Kim,and Young Jong Lee
Analytical Chemistry Published: September 4, 2024
DOI:https://doi.org/10.1021/acs.analchem.4c02108
Abstract
Absolute quantity imaging of biomolecules on a single cell level is critical for measurement assurance in biosciences and bioindustries. While infrared (IR) transmission microscopy is a powerful label-free imaging modality capable of chemical quantification, its applicability to hydrated biological samples remains challenging due to the strong IR absorption by water. Traditional IR imaging of hydrated cells relies on powerful light sources, such as synchrotrons, to mitigate the light absorption by water. However, we overcome this challenge by applying a solvent absorption compensation (SAC) technique to a home-built benchtop IR microscope based on an external-cavity quantum cascade laser. SAC-IR microscopy adjusts the incident light using a pair of polarizers to precompensate the IR absorption by water while retaining the full dynamic range. Integrating the IR absorbance over a cell yields the total mass of biomolecules per cell. We monitor the total mass of the biomolecules of live fibroblast cells over 12 h, demonstrating promise for advancing our understanding of the biomolecular processes occurring in live cells on the single-cell level.
