新たな成果は、様々な病原体や病気の検出に使用できる、より効率的で選択的、かつ高感度なDNAバイオセンサーのプラットフォームを提供するものです。 New results provide a platform for more efficient, selective, and sensitive DNA biosensors that can be used in detecting various pathogens and diseases.
2023-03-14 フィンランド・アールト大学
<関連情報>
- https://www.aalto.fi/en/news/dna-origami-boosts-electrochemical-biosensor-performance
- https://pubs.acs.org/doi/10.1021/acssensors.2c02469
標的捕捉型DNA折り紙タイルを用いた電気化学的DNAバイオセンサーの信号増幅法 Signal Amplification in Electrochemical DNA Biosensors Using Target-Capturing DNA Origami Tiles
Paul Williamson, Petteri Piskunen, Heini Ijäs, Adrian Butterworth, Veikko Linko and Damion K. Corrigan
ACS Sensors Published:March 13, 2023
DOI:https://doi.org/10.1021/acssensors.2c02469
Abstract
Electrochemical DNA (e-DNA) biosensors are feasible tools for disease monitoring, with their ability to translate hybridization events between a desired nucleic acid target and a functionalized transducer, into recordable electrical signals. Such an approach provides a powerful method of sample analysis, with a strong potential to generate a rapid time to result in response to low analyte concentrations. Here, we report a strategy for the amplification of electrochemical signals associated with DNA hybridization, by harnessing the programmability of the DNA origami method to construct a sandwich assay to boost charge transfer resistance (RCT) associated with target detection. This allowed for an improvement in the sensor limit of detection by two orders of magnitude compared to a conventional label-free e-DNA biosensor design and linearity for target concentrations between 10 pM and 1 nM without the requirement for probe labeling or enzymatic support. Additionally, this sensor design proved capable of achieving a high degree of strand selectivity in a challenging DNA-rich environment. This approach serves as a practical method for addressing strict sensitivity requirements necessary for a low-cost point-of-care device.
