2024-02-14 カリフォルニア大学校アーバイン校(UCI)
<関連情報>
- https://news.uci.edu/2024/02/14/uc-irvine-led-research-team-creates-novel-rabies-viral-vectors-for-neural-circuit-mapping/
- https://link.springer.com/article/10.1038/s41380-024-02451-6
マルチスケール神経回路マッピングのための新しい狂犬病ウイルス資源 New rabies viral resources for multi-scale neural circuit mapping
Alexis Bouin,Ginny Wu,Orkide O. Koyuncu,Qiao Ye,Keun-Young Kim,Michele Y. Wu,Liqi Tong,Lujia Chen,Sebastien Phan,Mason R. Mackey,Ranjan Ramachandra,Mark H. Ellisman,Todd C. Holmes,Bert L. Semler & Xiangmin Xu
Molecular Psychiatry Published:14 February 2024
DOI:https://doi.org/10.1038/s41380-024-02451-6
Abstract
Comparisons and linkage between multiple imaging scales are essential for neural circuit connectomics. Here, we report 20 new recombinant rabies virus (RV) vectors that we have developed for multi-scale and multi-modal neural circuit mapping tools. Our new RV tools for mesoscale imaging express a range of improved fluorescent proteins. Further refinements target specific neuronal subcellular locations of interest. We demonstrate the discovery power of these new tools including the detection of detailed microstructural changes of rabies-labeled neurons in aging and Alzheimer’s disease mouse models, live imaging of neuronal activities using calcium indicators, and automated measurement of infected neurons. RVs that encode GFP and ferritin as electron microscopy (EM) and fluorescence microscopy reporters are used for dual EM and mesoscale imaging. These new viral variants significantly expand the scale and power of rabies virus-mediated neural labeling and circuit mapping across multiple imaging scales in health and disease.
