腱板損傷後の筋肉を修復する双方向細胞ベースの治療法(Two-Way Cell-based Treatment Repairs Muscle After Rotator Cuff Injury)

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2024-03-26 ジョージア工科大学

腱板損傷後の筋肉を修復する双方向細胞ベースの治療法(Two-Way Cell-based Treatment Repairs Muscle After Rotator Cuff Injury)
A combination of mobilizing agent, designed to “push” pro-healing cells into the blood, and SDF-1a, designed to “pull” the cells into the injury site, leads to an increase in muscle regeneration following a rotator cuff tear. Muscle regeneration was characterized based on the number of centrally located nuclei (marked with the white arrows).

ジョージア工科大学の研究チームが、一般的なローテーターカフ手術によく見られる不良な臨床成果を補う新しい治療システムを導入しました。このシステムは、マイナーリーグ野球オールスターの投手クレイトン・カーショウやジャスティン・バーランダーのような有名アスリートが、ローテーターカフ手術で復帰した際にも注目されました。この治療法は、細胞をベースとした二重治療であり、ウォレスH. クーリエ生体医学工学部門の教授2人によって開発されました。研究はTissue Engineering誌に掲載されています。この治療法は、老化した人々によく見られる障害に焦点を当てており、広く使われる予定です。

<関連情報>

骨髄動員および局所間質細胞由来因子-1α導入が棘上筋線維の成長を促進する Bone Marrow Mobilization and Local Stromal Cell-Derived Factor-1α Delivery Enhances Nascent Supraspinatus Muscle Fiber Growth

Leah E. Anderson, Liane E. Tellier, Keshav R. Shah, Joseph J. Pearson, Alexandra L. Brimeyer, Edward A. Botchwey, and Johnna S. Temenoff
Tissue Engineering  Published:17 Jan 2024
DOI:https://doi.org/10.1089/ten.tea.2023.0128

Abstract

Rotator cuff tear is a significant problem that leads to poor clinical outcomes due to muscle degeneration after injury. The objective of this study was to synergistically increase the number of proregenerative cells recruited to injure rotator cuff muscle through a novel dual treatment system, consisting of a bone marrow mobilizing agent (VPC01091), hypothesized to “push” prohealing cells into the blood, and localized delivery of stromal cell-derived factor-1α (SDF-1α), to “pull” the cells to the injury site. Immediately after rotator cuff tendon injury in rat, the mobilizing agent was delivered systemically, and SDF-1α-loaded heparin-based microparticles were injected into the supraspinatus muscle. Regenerative and degenerative changes to supraspinatus muscle and the presence of inflammatory/immune cells, mesenchymal stem cells (MSCs), and satellite cells were assessed via flow cytometry and histology for up to 21 days. After dual treatment, significantly more MSCs (31.9 ± 8.0% single cells) and T lymphocytes (6.7 ± 4.3 per 20 × field of view) were observed in supraspinatus muscle 7 days after injury and treatment compared to injury alone (14.4 ± 6.5% single cells, 1.2 ± 0.7 per 20 × field of view), in addition to an elevated M2:M1 macrophage ratio (3.0 ± 0.5), an indicator of a proregenerative environment. These proregenerative cellular changes were accompanied by increased nascent fiber formation (indicated by embryonic myosin heavy chain staining) at day 7 compared to SDF-1α treatment alone, suggesting that this method may be a promising strategy to influence the early cellular response in muscle and promote a proregenerative microenvironment to increase muscle healing after severe rotator cuff tear.

医療・健康
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