2024-04-24 ミュンヘン大学(LMU)
<関連情報>
- https://www.lmu.de/en/newsroom/news-overview/news/biophysics-testing-how-well-biomarkers-work.html
- https://www.nature.com/articles/s41592-024-02242-5
単一タンパク質レベルでの絶対標識効率の定量化 Quantification of absolute labeling efficiency at the single-protein level
Joschka Hellmeier,Sebastian Strauss,Shuhan Xu,Luciano A. Masullo,Eduard M. Unterauer,Rafal Kowalewski & Ralf Jungmann
Nature Methods Published:24 April 2024
DOI:https://doi.org/10.1038/s41592-024-02242-5
Abstract
State-of-the-art super-resolution microscopy allows researchers to spatially resolve single proteins in dense clusters. However, accurate quantification of protein organization and stoichiometries requires a general method to evaluate absolute binder labeling efficiency, which is currently unavailable. Here we introduce a universally applicable approach that uses a reference tag fused to a target protein of interest. By attaching high-affinity binders, such as antibodies or nanobodies, to both the reference tag and the target protein, and then employing DNA-barcoded sequential super-resolution imaging, we can correlate the location of the reference tag with the target molecule binder. This approach facilitates the precise quantification of labeling efficiency at the single-protein level.
