神経細胞の劇的な変化の詳細な青写真は、脊髄損傷を治癒する方法の特定に役立つ可能性がある(Detailed blueprint of nerve cells’ dramatic changes could help identify ways to heal spinal cord damage)

2024-08-15 ワシントン大学セントルイス校

<関連情報>

• https://source.washu.edu/2024/08/zebrafish-use-surprising-strategy-to-regrow-spinal-cord/
• https://www.nature.com/articles/s41467-024-50628-y

自然発生的脊髄再生の単一細胞解析から、神経細胞修復の交差様式が明らかになった Single-cell analysis of innate spinal cord regeneration identifies intersecting modes of neuronal repair

Vishnu Muraleedharan Saraswathy,Lili Zhou & Mayssa H. Mokalled
Nature Communications  Published:15 August 2024
DOI:https://doi.org/10.1038/s41467-024-50628-y

Abstract

Adult zebrafish have an innate ability to recover from severe spinal cord injury. Here, we report a comprehensive single nuclear RNA sequencing atlas that spans 6 weeks of regeneration. We identify cooperative roles for adult neurogenesis and neuronal plasticity during spinal cord repair. Neurogenesis of glutamatergic and GABAergic neurons restores the excitatory/inhibitory balance after injury. In addition, a transient population of injury-responsive neurons (iNeurons) show elevated plasticity 1 week post-injury. We found iNeurons are injury-surviving neurons that acquire a neuroblast-like gene expression signature after injury. CRISPR/Cas9 mutagenesis showed iNeurons are required for functional recovery and employ vesicular trafficking as an essential mechanism that underlies neuronal plasticity. This study provides a comprehensive resource of the cells and mechanisms that direct spinal cord regeneration and establishes zebrafish as a model of plasticity-driven neural repair.