2025-04-03 オークリッジ国立研究所 (ORNL)
<関連情報>
- https://www.ornl.gov/news/novel-biosensor-illuminates-plant-rna-real-time
- https://onlinelibrary.wiley.com/doi/10.1111/pbi.14612
in vivo植物RNAイメージングと遺伝子工学のための分割リボザイムシステム A split ribozyme system for in vivo plant RNA imaging and genetic engineering
Yang Liu, Ruchika Rajput, Md Torikul Islam, Ilenne Del Valle, Tao Yao, Rekha Agrawal, Brandon A. Boone, Carrie A. Eckert, Paul E. Abraham, Jin-Gui Chen, Gerald A. Tuskan, Xiaohan Yang
Plant Biotechnology Journal Published: 07 February 2025
DOI:https://doi.org/10.1111/pbi.14612
Summary
RNA plays a central role in plants, governing various cellular and physiological processes. Monitoring its dynamic abundance provides a discerning understanding of molecular mechanisms underlying plant responses to internal (developmental) and external (environmental) stimuli, paving the way for advances in plant biotechnology to engineer crops with improved resilience, quality and productivity. In general, traditional methods for analysis of RNA abundance in plants require destructive, labour-intensive and time-consuming assays. To overcome these limitations, we developed a transformative innovation for in vivo RNA imaging in plants. Specifically, we established a synthetic split ribozyme system that converts various RNA signals to orthogonal protein outputs, enabling in vivo visualisation of various RNA signals in plants. We demonstrated the utility of this system in transient expression experiments (i.e., leaf infiltration in Nicotiana benthamiana) to detect RNAs derived from transgenes and tobacco rattle virus, respectively. Also, we successfully engineered a split ribozyme-based biosensor in Arabidopsis thaliana for in vivo visualisation of endogenous gene expression at the cellular level, demonstrating the feasibility of multi-scale (e.g., cellular and tissue level) RNA imaging in plants. Furthermore, we developed a platform for easy incorporation of different protein outputs, allowing for flexible choice of reporters to optimise the detection of target RNAs.
