2025-07-11 テキサスA&M大学
Texas A&M researchers created a system that allows high throughput screening of molecules and cells at significantly reduced error rates. Credit: Danielle Benavides/Texas A&M Engineering
<関連情報>
- https://stories.tamu.edu/news/2025/07/11/unlocking-the-future-of-next-generation-cancer-drugs/
- https://www.nature.com/articles/s41467-024-52932-z
エラーのない液滴マイクロ流体工学のためのNOVAsort NOVAsort for error-free droplet microfluidics
Han Zhang,Rohit Gupte,Yuwen Li,Can Huang,Adrian R. Guzman,Jeong Jae Han,Haemin Jung,Rushant Sabnis,Paul de Figueiredo & Arum Han
Nature Communications Published:01 November 2024
DOI:https://doi.org/10.1038/s41467-024-52932-z
Abstract
High-throughput screening techniques are pivotal to unlocking the mysteries of biology. Yet, the promise of droplet microfluidics in enabling single-cell resolution, ultra-high-throughput screening remains largely unfulfilled. Droplet sorting errors caused by polydisperse droplet sizes that are often inevitable in multi-step assays have severely limited the effectiveness and utility of this technique, especially when screening large libraries. Even a relatively low 1% sorting error results in 10,000 false calls in a 1,000,000 droplet screen, imposing an unreasonably large burden on downstream validation. Here, we present NOVAsort (Next-generation Opto-Volume-based Accurate droplet sorter), a device capable of discerning droplets based on both size and fluorescence intensity. With a 1000- and 10,000-fold reduction in false positives and false negatives, respectively. NOVAsort addresses the challenges of conventional droplet sorting approaches and sets standards for accuracy and throughput in droplet microfluidic assays.
