2025-07-15 中国科学院(CAS)
<関連情報>
- https://english.cas.cn/newsroom/research_news/life/202507/t20250711_1047212.shtml
- https://www.cell.com/cell/abstract/S0092-8674(25)00639-7
ニホンザル前頭前野の単一ニューロンプロジェクトームから明らかになった精緻な軸索ターゲティングと樹状化 Single-neuron projectomes of macaque prefrontal cortex reveal refined axon targeting and arborization
Lingfeng Gou ∙ Yanzhi Wang ∙ Le Gao ∙ … ∙ Zhiming Shen ∙ Chun Xu ∙ Jun Yan
Cell Published:June 3, 2025
DOI:https://doi.org/10.1016/j.cell.2025.06.005
Graphical abstract
Highlights
- 2,231 single-neuron projectomes were reconstructed for the macaque PFC
- 32 neuron subtypes were classified and their putative functions annotated
- An intra-PFC connectivity network and extensive local axons were uncovered
- Macaque PFC neurons have more refined targeting and axon arbors than mouse neurons
Summary
Cortical expansion endows advanced cognitive functions in primates, and whole-brain single-neuron projection analysis helps to elucidate underlying neural circuit mechanisms. Here, we reconstructed 2,231 single-neuron projectomes for the macaque prefrontal cortex (PFC) and identified 32 projectome-based subtypes of intra-telencephalic, pyramidal-tract, and cortico-thalamic neurons. Each subtype exhibited distinct topography in their soma distribution within the PFC, a characteristic pattern of axon targeting, and subregion-specific patchy terminal arborization in the targeted area, with putative functions annotated. Furthermore, we identified a subdomain connectivity network and extensive local axons within the PFC. Compared with those in mice, macaque PFC projectomes exhibited a similar topographic gradient of terminal arborization at the targeted regions but much higher target specificity, fewer collaterals, and smaller brain size-normalized arbors. Thus, whole-brain single-axon macaque projectomes revealed highly refined axon targeting and arborization, providing key insights into the structural basis for complex brain functions in primates.
