2025-07-31 シカゴ大学(UChicago)
<関連情報>
- https://news.uchicago.edu/story/innovative-liquid-biopsy-test-uses-rna-detect-early-stage-cancer
- https://www.nature.com/articles/s41587-025-02731-8
- https://www.nature.com/articles/s41477-023-01580-0
マイクロバイオーム由来の細胞フリーRNAの血漿中の修飾が結腸直腸がんサンプルを区別する Modifications of microbiome-derived cell-free RNA in plasma discriminates colorectal cancer samples
Cheng-Wei Ju,Ruitu Lyu,Han Li,Jiangbo Wei,Alberto J. Parra Vitela,Urszula Dougherty,Akushika Kwesi,Alexander Luna,Xuanhao Zhu,Shenghai Shen,Yunzheng Liu,Liangliang Wang,Xiaolong Cui,Yuzhi Xu,Bochen Jiang,Yiyi Ji,Peng Xia,Diana C. West-Szymanski,Chenxi Sun,Yuhao Zhong,Chang Ye,Angelica Moran,Christopher Lehmann,Eric Pamer,… Chuan He
Nature Biotechnology Published:08 July 2025
DOI:https://doi.org/10.1038/s41587-025-02731-8
Abstract
Circulating cell-free RNA (cfRNA) in plasma represents a promising avenue for cancer detection. We report low-input multiple methylation sequencing, a method for profiling modification patterns in cfRNA, enabling the detection of diverse transfer RNAs and small noncoding RNAs derived from both the human genome and the microbiome. RNA modification patterns in microbiome-derived cfRNA accurately reflect host microbiota activity and hold potential for the early detection of colorectal cancer.
アラビドプシスにおけるmRNAのメチル化とクロロフィル恒常性を制御するCRY2/SPA1/FIO1複合体の光誘導性LLPS Light-induced LLPS of the CRY2/SPA1/FIO1 complex regulating mRNA methylation and chlorophyll homeostasis in Arabidopsis
Bochen Jiang,Zhenhui Zhong,Lianfeng Gu,Xueyang Zhang,Jiangbo Wei,Chang Ye,Guifang Lin,Gaoping Qu,Xian Xiang,Chenjin Wen,Maureen Hummel,Julia Bailey-Serres,Qin Wang,Chuan He,Xu Wang & Chentao Lin
Nature Plants Published:08 December 2023
DOI:https://doi.org/10.1038/s41477-023-01580-0
Abstract
Light regulates chlorophyll homeostasis and photosynthesis via various molecular mechanisms in plants. The light regulation of transcription and protein stability of nuclear-encoded chloroplast proteins have been extensively studied, but how light regulation of mRNA metabolism affects abundance of nuclear-encoded chloroplast proteins and chlorophyll homeostasis remains poorly understood. Here we show that the blue light receptor cryptochrome 2 (CRY2) and the METTL16-type m6A writer FIONA1 (FIO1) regulate chlorophyll homeostasis in response to blue light. In contrast to the CRY2-mediated photo-condensation of the mRNA adenosine methylase (MTA), photoexcited CRY2 co-condenses FIO1 only in the presence of the CRY2-signalling protein SUPPRESSOR of PHYTOCHROME A (SPA1). CRY2 and SPA1 synergistically or additively activate the RNA methyltransferase activity of FIO1 in vitro, whereas CRY2 and FIO1, but not MTA, are required for the light-induced methylation and translation of the mRNAs encoding multiple chlorophyll homeostasis regulators in vivo. Our study demonstrates that the light-induced liquid–liquid phase separation of the photoreceptor/writer complexes is commonly involved in the regulation of photoresponsive changes of mRNA methylation, whereas the different photo-condensation mechanisms of the CRY/FIO1 and CRY/MTA complexes explain, at least partially, the writer-specific functions in plant photomorphogenesis.
