2025-10-03 基礎生物学研究所
図1 本研究で明らかになった植物における液胞膜やエンドソームからの膜タンパク質回収経路。二つの独立した膜タンパク質回収経路(オレンジ色と青色)が液胞やエンドソームの独自の膜タンパク質組成をつくり出している。
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植物におけるSNAREの新機能化を支える液胞およびエンドソーム区画からの回収 Retrieval from vacuolar and endosomal compartments underpinning the neofunctionalization of SNARE in plants
Yihong Feng,Kazuo Ebine,Yoko Ito,Takehiko Kanazawa,Tatsuya Sawasaki,Akira Nozawa,Tomohiro Uemura,Akihiko Nakano & Takashi Ueda
Nature Plants Published:03 October 2025
DOI:https://doi.org/10.1038/s41477-025-02115-5
Abstract
A distinctive protein and lipid composition underlies the distinct function of each organelle, regulated by balanced anterograde and retrograde membrane trafficking. The vacuole, the largest plant organelle, is pivotal in various plant functions, and its protein composition is tightly regulated by bidirectional trafficking. However, the existence of retrograde transport from the plant vacuole has remained unverified. Here we demonstrate retrograde trafficking from the vacuole in Arabidopsis. We observed the retrieval of VAMP727, a plant-unique vacuolar membrane fusion machinery, from the vacuolar membrane. VAMP727 retrieval is facilitated by sorting nexin proteins, which independently diversified between plant and non-plant systems. Furthermore, we show that the core retromer complex and sorting nexins act independently in distinct retrograde transport events with specific cargos. Plant cells have thus elaborated a unique retrieval mechanism from the vacuole, underpinning the neofunctionalization of VAMP727 during plant evolution.
