2024-10-23 ニューヨーク大学 (NYU)
<関連情報>
- https://www.nyu.edu/about/news-publications/news/2024/october/cancer-metastasis-3mic.html
- https://www.life-science-alliance.org/content/8/1/e202403053
腫瘍微小環境の生体外モデル内で出現する転移の特徴を直接可視化する Direct visualization of emergent metastatic features within an ex vivo model of the tumor microenvironment
Libi Anandi, Jeremy Garcia, Manon Ros, Libuše Janská, Josephine Liu,Carlos Carmona-Fontaine
Life Science Alliance Published 17 October 2024
DOI: 10.26508/lsa.202403053
Abstract
Ischemic conditions such as hypoxia and nutrient starvation, together with interactions with stromal cells, are critical drivers of metastasis. These conditions arise deep within tumor tissues, and thus, observing nascent metastases is exceedingly challenging. We thus developed the 3MIC—an ex vivo model of the tumor microenvironment—to study the emergence of metastatic features in tumor cells in a 3-dimensional (3D) context. Here, tumor cells spontaneously create ischemic-like conditions, allowing us to study how tumor spheroids migrate, invade, and interact with stromal cells under different metabolic conditions. Consistent with previous data, we show that ischemia increases cell migration and invasion, but the 3MIC allowed us to directly observe and perturb cells while they acquire these pro-metastatic features. Interestingly, our results indicate that medium acidification is one of the strongest pro-metastatic cues and also illustrate using the 3MIC to test anti-metastatic drugs on cells experiencing different metabolic conditions. Overall, the 3MIC can help dissecting the complexity of the tumor microenvironment for the direct observation and perturbation of tumor cells during the early metastatic process.
