2004-10-24 カリフォルニア大学校アーバイン校(UCI)
<関連情報>
- https://news.uci.edu/2024/10/24/uc-irvine-scientists-uncovering-link-between-gut-e-coli-and-parkinsons-disease/
- https://pubs.acs.org/doi/10.1021/acschemneuro.4c00106
- https://pubs.acs.org/doi/10.1021/acschembio.4c00095
レーザープリンティング電気化学センサーを用いたパーキンソン病における腸内αシヌクレイン凝集のメカニズム解明 Mechanistic Insight into Intestinal α-Synuclein Aggregation in Parkinson’s Disease Using a Laser-Printed Electrochemical Sensor
Julia M. Balsamo,Keren Zhou,Vinay Kammarchedu,Aida Ebrahimi,and Elizabeth N. Bess
ACS Chemical Neuroscience Published: July 3, 2024
DOI:https://doi.org/10.1021/acschemneuro.4c00106
Abstract
Aggregated deposits of the protein α-synuclein and depleting levels of dopamine in the brain correlate with Parkinson’s disease development. Treatments often focus on replenishing dopamine in the brain; however, the brain might not be the only site requiring attention. Aggregates of α-synuclein appear to accumulate in the gut years prior to the onset of any motor symptoms. Enteroendocrine cells (specialized gut epithelial cells) may be the source of intestinal α-synuclein, as they natively express this protein. Enteroendocrine cells are constantly exposed to gut bacteria and their metabolites because they border the gut lumen. These cells also express the dopamine metabolic pathway and form synapses with vagal neurons, which innervate the gut and brain. Through this connection, Parkinson’s disease pathology may originate in the gut and spread to the brain over time. Effective therapeutics to prevent this disease progression are lacking due to a limited understanding of the mechanisms by which α-synuclein aggregation occurs in the gut. We previously proposed a gut bacterial metabolic pathway responsible for the initiation of α-synuclein aggregation that is dependent on the oxidation of dopamine. Here, we develop a new tool, a laser-induced graphene-based electrochemical sensor chip, to track α-synuclein aggregation and dopamine level over time. Using these sensor chips, we evaluated diet-derived catechols dihydrocaffeic acid and caffeic acid as potential inhibitors of α-synuclein aggregation. Our results suggest that these molecules inhibit dopamine oxidation. We also found that these dietary catechols inhibit α-synuclein aggregation in STC-1 enteroendocrine cells. These findings are critical next steps to reveal new avenues for targeted therapeutics to treat Parkinson’s disease, specifically in the context of functional foods that may be used to reshape the gut environment.
α-シヌクレインの凝集を促進する腸内細菌代謝経路を発見 Discovery of a Gut Bacterial Metabolic Pathway that Drives α-Synuclein Aggregation
Lizett Ortiz de Ora,Julia M. Balsamo,Kylie S. Uyeda,and Elizabeth N. Bess
ACS Chemical Biology Published: March 22, 2024
DOI:https://doi.org/10.1021/acschembio.4c00095
Abstract
Parkinson’s disease (PD) etiology is associated with aggregation and accumulation of α-synuclein (α-syn) proteins in midbrain dopaminergic neurons. Emerging evidence suggests that in certain subtypes of PD, α-syn aggregates originate in the gut and subsequently spread to the brain. However, mechanisms that instigate α-syn aggregation in the gut have remained elusive. In the brain, the aggregation of α-syn is induced by oxidized dopamine. Such a mechanism has not been explored in the context of the gastrointestinal tract, a niche harboring 46% of the body’s dopamine reservoirs. Here, we report that Enterobacteriaceae, a bacterial family prevalent in human gut microbiotas, induce α-syn aggregation. More specifically, our in vitro data indicate that respiration of nitrate by Escherichia coli K-12, which results in production of nitrite that mediates oxidation of Fe2+ to Fe3+, creates an oxidizing redox potential. These oxidizing conditions enabled the formation of dopamine-derived quinones and α-syn aggregates. Exposing nitrite, but not nitrate, to enteroendocrine STC-1 cells induced aggregation of α-syn that is natively expressed in these cells, which line the intestinal tract. Taken together, our findings indicate that bacterial nitrate reduction may be critical for initiating intestinal α-syn aggregation.
