2026-02-06 カリフォルニア大学サンディエゴ校(UCSD)
<関連情報>
- https://today.ucsd.edu/story/next-generation-genetics-technology-developed-to-counter-the-rise-of-antibiotic-resistance
- https://www.nature.com/articles/s44259-026-00181-z
接合遺伝子ドライブのようなシステムが細菌集団における抗生物質耐性を効率的に抑制する A conjugal gene drive-like system efficiently suppresses antibiotic resistance in a bacterial population
Saluja Kaduwal,Elizabeth C. Stuart,Ankush Auradkar,Seth Washabaugh,Justin R. Meyer & Ethan Bier
npj Antimicrobials and Resistance Published:02 February 2026
DOI:https://doi.org/10.1038/s44259-026-00181-z
Abstract
Antibiotic resistance (AR) is an escalating public health threat, necessitating innovative strategies to control resistant bacterial populations. One promising approach involves engineering genetic elements that can spread within microbial communities to eliminate AR genes. Previously, we developed Pro-Active Genetics (Pro-AG), a CRISPR-based gene-drive-like system capable of reducing AR colony-forming units (CFU) by approximately five logs. Here, we advance this technology by integrating Pro-AG into a conjugative transfer system, enabling efficient dissemination of an anti-AR gene cassette between two bacterial strains. Additionally, we characterize a complementary homology-based deletion (HBD) process, a CRISPR-driven mechanism that precisely removes target DNA sequences flanked by short direct repeats. Our findings reveal that Pro-AG and HBD are differentially influenced by the bacterial RecA pathway and that HBD components can be delivered via plasmids or phages to selectively delete Pro-AG cassettes. This built-in safeguard prevents uncontrolled spread of a gene cassette and mitigates unanticipated side effects. These refinements enhance the efficiency and flexibility of Pro-AG, expanding its potential applications in microbiome engineering, environmental remediation, and clinical interventions aimed at combating antibiotic resistance. More broadly, this work establishes a proof-of-principle for microbiome engineering strategies that could be leveraged to improve health and restore ecological balance.
